FlyBase Allele Report: Dmel\mir-318[Δ2]

General Information

Symbol

Dmel\mir-318^Δ2

Species

D. melanogaster

Name

FlyBase ID

FBal0304753

Feature type

allele

Associated gene

Dmel\mir-318

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

amorphic allele - molecular evidence

Mutagen

P-element activity

Nature of the Allele

Allele class

amorphic allele - molecular evidence

(Ge et al., 2015)

Mutagen

P-element activity

(Ge et al., 2015)

Progenitor genotype

P{GSV6}GS15934

(Ge et al., 2015)

Cytology

Description

Imprecise excision of P{GSV6}GS15934 has resulted in a 1.8kb deletion.

(Ge et al., 2015)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

deletion

3R:10,407,286..10,409,088 [+]

Comment:

A 1.8 kb deletion resulting from the imprecise excision of P{GSV6}GS15934, which removes mir-318. The deletion is reported to remove sequences between R6 coordinates 3R:10 ,407,286..10,409,088.

(Ge et al., 2015)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

majority die during embryonic stage (with mir-318^Δ1)

(Ge et al., 2015)

viable (with mir-318^Δ1)

(Ge et al., 2015)

Phenotype Manifest In

dorsal appendage (with mir-318^Δ1)

(Ge et al., 2015)

egg (with mir-318^Δ1)

(Ge et al., 2015)

egg operculum (with mir-318^Δ1)

(Ge et al., 2015)

follicle cell (with mir-318^Δ1)

(Ge et al., 2015)

Detailed Description

Statement

Reference

mir-318^Δ1/mir-318^Δ2 mutant flies are fully viable and do not show obvious morphological defects. However, mir-318^Δ1/mir-318^Δ2 mutant females lay eggs with a range of defects in eggshell formation and patterning. More than 90% of eggs from mir-318^Δ1/mir-318^Δ2 mutant mothers show defects in dorsal pattern: the dorsal appendages are shifted towards posterior and are closer together than in wild type embryos. The operculum is slightly enlarged.

76% of eggs from mir-318^Δ1/mir-318^Δ2 mutant mothers fail to hatch into first instar larvae. Most of the eggs appear not to be fertilized, and the micropyle is reduced or absent. Eggs also have a transparent and thin eggshell. 12% of eggs collapse by 48 hours after laying.

mir-318^Δ1/mir-318^Δ2 mutants females show defects in follicle cell patterning. The transition from endocycles to chorion gene amplification is compromised in mir-318^Δ1/mir-318^Δ2 mutant follicle cells.

(Ge et al., 2015)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Other

Statement

Reference

mir-318^Δ1/mir-318^Δ2, ttk[+]/ttk^1e11 has lethal - all die during embryonic stage phenotype

(Ge et al., 2015)

Df(3R)ED6361/+, mir-318^Δ1/mir-318^Δ2 has lethal - all die during embryonic stage phenotype

(Ge et al., 2015)

Phenotype Manifest In

Enhanced by

Statement

Reference

mir-318^Δ1/mir-318^Δ2 has egg phenotype, enhanceable by ttk[+]/ttk^1e11

(Ge et al., 2015)

mir-318^Δ1/mir-318^Δ2 has egg phenotype, enhanceable by Df(3R)ED6361/+

(Ge et al., 2015)

Additional Comments

Genetic Interactions

Statement

Reference

One copy of ttk^1e11 enhances the abnormal egg phenotype seen in mir-318^Δ1/mir-318^Δ2 mutants, including short dorsal appendages. None of the mir-318^Δ1 mir-318^Δ2/ttk^1e11 mutant flies are able to hatch into first instar larval females. A more severe reduction in chorion gene amplification is seen.

One copy of Df(3R)ED6361 enhances the abnormal egg phenotype seen in mir-318^Δ1/mir-318^Δ2 mutants, including short dorsal appendages. None of the mir-318^Δ1 mir-318^Δ2/Df(3R)ED6361 mutant flies are able to hatch into first instar larval females.

(Ge et al., 2015)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

mir-318^Δ1/mir-318^Δ2 is rescued by mir-318^+tGa

(Ge et al., 2015)

Comments

Expression of mir-318^+tGa rescues the defects seen in eggs laid by mir-318^Δ1/mir-318^Δ2 mutant mothers. The hatching rate is restored to wild type levels.

(Ge et al., 2015)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

mir-318^Δ2

(Ge et al., 2015)

Name Synonyms

Secondary FlyBase IDs

References (1)

Report Sections

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