FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\Osi21die4
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General Information
Symbol
Dmel\Osi21die4
Species
D. melanogaster
Name
FlyBase ID
FBal0318438
Feature type
allele
Associated gene
Dmel\Osi21
Associated Insertion(s)
Carried in Construct
Also Known As
diehard4
Key Links
Genomic Maps

Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
ethyl methanesulfonate
(Lee, 2012)
Progenitor genotype
Cytology
Description

Amino acid replacement: G149S.

(Lee et al., 2013)

Amino acid replacement: M181T.

(Lee et al., 2013)

Amino acid replacement: F229L.

(Lee et al., 2013)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
point_mutation
2L:11,284,919..11,284,919 [-]
Nucleotide change:

T11284919C

Amino acid change:

F229L | Osi21-PA

Reported amino acid change:

F229L

Comment:

A nucleotide change at the first or third position of the Phe codon leads to a Leu mutation (base change unspecified).

(Lee et al., 2013)
point_mutation
2L:11,285,062..11,285,062 [-]
Nucleotide change:

T11285062C

Amino acid change:

M181T | Osi21-PA

Reported amino acid change:

M181T

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(Lee et al., 2013)
point_mutation
2L:11,285,159..11,285,159 [-]
Nucleotide change:

G11285159A

Amino acid change:

G149S | Osi21-PA

Reported amino acid change:

G149S

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(Lee et al., 2013)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Suppressor of
      Statement
      Reference

      Osi21MB01450/Osi21die4 is a suppressor of increased cell death | conditional phenotype of norpAP24

      (Lee et al., 2013)

      Osi21die4/Osi21die4 is a suppressor of increased cell death | conditional phenotype of rdgC306

      (Lee et al., 2013)

      Osi21die4/Osi21die4 is a suppressor of increased cell death | conditional phenotype of trp1

      (Lee et al., 2013)
      NOT Suppressor of
      Statement
      Reference

      Osi21die4/Osi21die4 is a non-suppressor of increased cell death | conditional phenotype of rdgB2

      (Lee et al., 2013)
      Other
      Statement
      Reference

      Osi21+tLa, Osi21MB01450/Osi21die4, norpAP24 has increased cell death | conditional phenotype

      (Lee et al., 2013)
      Phenotype Manifest In
      Suppressor of
      Statement
      Reference

      Osi21MB01450/Osi21die4 is a suppressor of retina | conditional phenotype of norpAP24

      (Lee et al., 2013)

      Osi21die4/Osi21die4 is a suppressor of retina | conditional phenotype of rdgC306

      (Lee et al., 2013)

      Osi21die4/Osi21die4 is a suppressor of retina | conditional phenotype of trp1

      (Lee et al., 2013)
      NOT Suppressor of
      Statement
      Reference

      Osi21die4/Osi21die4 is a non-suppressor of retina | conditional phenotype of rdgB2

      (Lee et al., 2013)
      Other
      Statement
      Reference

      Osi21+tLa, Osi21MB01450/Osi21die4, norpAP24 has retina | conditional phenotype

      (Lee et al., 2013)
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Osi21die4/Osi21die4, Osi21die4/Df(2L)Exel6028 or Osi21die4/Osi21MB01450 (but not Osi21die4/Osi21MB01450;Osi21+tLa) partially suppresses the progressive retinal degeneration seen under constant light exposure in norpA36/norpA36 flies. Osi21die4/Osi21die4 partially suppresses the progressive retinal degeneration seen under constant light exposure in trp1/trp1 or rdgC306/rdgC306 (but not rdgB2/rdgB2) flies.

      (Lee et al., 2013)

      The retinal degeneration (loss of deep pseudopupil) seen in norpA36 flies after 5 days in constant light is suppressed if the eyes are homozygous for diehard4diehard4.

      (Lee, 2012)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer

      Separable from: a secondary lethal mutation; the chromosome is homozygous lethal, but is viable over a deficiency (Df(2L)Exel6028) that uncovers Osi21 (the Osi21die4/Df(2L)Exel6028 combination shows the suppression of norpA36 retinal degeneration, as expected).

      (Lee et al., 2013)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (4)
      Reported As
      Symbol Synonym
      Osi21die4
      die4
      (Lee et al., 2013)
      diehard4
      (Lee, 2012)
      diehard4diehard4
      Name Synonyms
      diehard4
      (Lee et al., 2013)
      Secondary FlyBase IDs
        References (2)