FlyBase Allele Report: Dmel\Sse[dft]

General Information

Symbol

Dmel\Sse^dft

Species

D. melanogaster

Name

FlyBase ID

FBal0318601

Feature type

allele

Associated gene

Dmel\Sse

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

hypomorphic allele - molecular evidence

Mutagen

Nature of the Allele

Allele class

hypomorphic allele - molecular evidence

(Cipressa et al., 2016)

Mutagen

Progenitor genotype

Cytology

Description

3bp deletion, which results in the loss of residues Phe265 and Gly266 and their replacement by a Cys residue.

(Cipressa et al., 2016)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

deletion

3L:5,661,155..5,661,157 [-]

Comment:

3 bp deletion (TTG) at position 794 from the initiation codon. Phe265 and Gly266 are replaced with a Cys. The resulting protein which is shorter by a single amino acid is not functional.

(Cipressa et al., 2016)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal endomitotic cell cycle | larval stage

(Cipressa et al., 2016)

abnormal endomitotic cell cycle | larval stage (with Df(3L)ZN47)

(Cipressa et al., 2016)

abnormal endomitotic cell cycle | larval stage (with Sse^13m)

(Cipressa et al., 2016)

Phenotype Manifest In

chromosome | larval stage

(Cipressa et al., 2016)

chromosome | larval stage (with Df(3L)ZN47)

(Cipressa et al., 2016)

chromosome | larval stage (with Sse^13m)

(Cipressa et al., 2016)

larval neuroblast | larval stage

(Cipressa et al., 2016)

larval neuroblast | larval stage (with Df(3L)ZN47)

(Cipressa et al., 2016)

larval neuroblast | larval stage (with Sse^13m)

(Cipressa et al., 2016)

Detailed Description

Statement

Reference

Sse^dft/Sse^dft, Sse^dft/Sse^13m or Sse^dft/Df(3L)ZN47 larval brains have telomeric fusions and endoreduplication phenotypes: neuroblasts have few dividing cells (around 3 metaphases per brain) and most cells are endoreduplicated (showing bundles of 2, 4, and 8 sister chromosomes and ploidy levels from 4n to 32n), and a large number of metaphases contain 64 (16n) or more than 64 chromosomes; some endoreduplicated chromosomes are also fused at their ends, giving rise to multicentric chromosome configurations and rings. Telomeric fusion frequency is increased (ratio between number of double telomere attachments and total number of chromosomes, which in mutants does not change significantly with the degree of endoreduplication).

(Cipressa et al., 2016)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Sse^dft has abnormal endomitotic cell cycle | larval stage phenotype, suppressible | partially by Su(var)205^g.mRFP(Unk)

(Cipressa et al., 2016)

Phenotype Manifest In

Suppressed by

Statement

Reference

Sse^dft has larval neuroblast | larval stage phenotype, suppressible | partially by Su(var)205^g.mRFP(Unk)

(Cipressa et al., 2016)

Additional Comments

Genetic Interactions

Statement

Reference

Su(var)205^g.T:Disc\RFP partially suppresses the telomeric fusion phenotype (but not endoreduplication) seen in Sse^dft/Sse^dft larval brain neuroblasts.

(Cipressa et al., 2016)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Sse^dft is rescued by Sse^UAS.Tag:HA/Scer\GAL4^69B

(Cipressa et al., 2016)

Not rescued by

Sse^dft is not rescued by Sse^{C497S.UAS.Tag:HA}/Scer\GAL4^69B

(Cipressa et al., 2016)

Comments

Expression of Sse^{Scer\UAS.T:Ivir\HA1} driven by Scer\GAL4^69B rescues telomeric fusions and endoreduplication phenotypes in Sse^dft/Sse^dft larval brain neuroblasts.

(Cipressa et al., 2016)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Separable from: His2Av⁰⁵¹⁴⁶.

(Cipressa et al., 2016)

The "l(3)05146" chromosome carries two separate mutations: His2Av⁰⁵¹⁴⁶ and the second site mutation Sse^dft.

(Cipressa et al., 2016)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Sse^dft

(Cipressa et al., 2025, Cipressa et al., 2016)

dft

(Cipressa et al., 2016)

Name Synonyms

diplofusedtelomeres

(Cipressa et al., 2016)

Secondary FlyBase IDs

References (2)

Report Sections

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