FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Mmus\PrnpFFI.3F4.UAS
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General Information
Symbol
Mmus\PrnpFFI.3F4.UAS
Species
M. musculus
Name
FlyBase ID
FBal0343118
Feature type
allele
Associated gene
Mmus\Prnp
Associated Insertion(s)
Carried in Construct
M{UAS-Mmus\Prnp.3F4.FFI}
Transgenic product class
Mutagen
Nature of the Allele
Transgenic product class
Mutagen
in vitro construct
(Thackray et al., 2017)
Progenitor genotype
Carried in construct
M{UAS-Mmus\Prnp.3F4.FFI}
Cytology
Description

UASt drives expression of the mature Mmus\Prnp 3F4 protein (the 3F4 epitope is created via two amino acid substitutions, L108M and V111M, and is already present in wild-type human and hamster PrP) carrying a single-codon mutation (D177N) associated with fatal familial insomnia (FFI) prion disease in humans, flanked by the coding sequence of its autologous leader peptide and GPI signal sequence.

(Thackray et al., 2017)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
Mmus\Prnp
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 1 )
      Disease
      Evidence
      References
      model of  prion disease
      CEA with Mmus\PrnpWT.3F4.UAS
      (Thackray et al., 2017)
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      This allele represents a human variant implicated in disease.
      (Thackray et al., 2017)

      Mouse gene.

      PRNP:p.Asp178Asn
      (FlyBase curators, 2019-)
      Variants Synonym(s)
      Associated human disease model(s)
      External database links
      ClinVar: PRNP_39359
      Comments concerning this variant
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      The expression of Mmus\PrnpFFI.3F4.UAS under the control of Scer\GAL4elav-C155 leads to adults showing a progressive decrease in climbing ability.

      The progressive decrease in climbing ability induced by the expression of Mmus\PrnpWT.3F4.UAS under the control of Scer\GAL4elav-C155 is enhanced by inoculation with brain extracts from individuals expressing Mmus\PrnpFFI.3F4.UAS under the control of Scer\GAL4elav-C155.

      (Thackray et al., 2017)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      Mmus\PrnpFFI.3F4.UAS
      Name Synonyms
      Secondary FlyBase IDs
        References (2)