FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\Diap1Ubi-p63E.DQVA.G4
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General Information
Symbol
Dmel\Diap1Ubi-p63E.DQVA.G4
Species
D. melanogaster
Name
FlyBase ID
FBal0363897
Feature type
allele
Associated gene
Dmel\Diap1
Associated Insertion(s)
Carried in Construct
P{Ubi-p63E-mCD8::DQVA::G4}
Key Links
Transgenic product class
Mutagen
Nature of the Allele
Transgenic product class
Mutagen
in vitro construct
(Tang et al., 2015)
Progenitor genotype
Carried in construct
P{Ubi-p63E-mCD8::DQVA::G4}
Cytology
Description

The Ubi-p63E promoter drives expression of a fusion protein comprised of a transmembrane domain (Tag:M(mCd8a)), amino acids 2-147 of Diap1, GAL4 and a Tag:MYC tag. The caspase cleavage site in the Diap1 sequence has been mutated to DQVAGV, so that it can no longer be cleaved. The Diap1 sequence also includes the a D135R mutation. The transmembrane domain tethers the fusion protein to the membrane regardless of caspase activity, preventing the GAL4 sequence from entering the nucleus, and thus it is unable to drive expression of UAS-regulated responder transgenes. This transgene is intended as a control for the Caspase-sensitive P{Ubi-p63E-mCD8::DQVD::GAL4} sensor.

(Tang et al., 2015)
Allele components
Component
Use(s)
Regulatory region(s)
Ubi-p63E
Encoded product / tool
Diap1
Tagged with
Tag:M(mCd8a)
Tag:MYC
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      Diap1Ubi-p63E.DQVA.G4
      Name Synonyms
      Secondary FlyBase IDs
        References (1)