No expression of the lexA reporter sequence inserted in TI{TI}Rh501 has been detected, likely because it is not near the promoter region of Rh50.
Deletion that removes the terminal 143 amino acid residues (29%) of the Rh50 coding sequence, including several transmembrane domains. Sequence from the donor plasmid, including an ampicillin resistance region, a lexA reporter and a Disc\RFPDsRed.3xP3.cUa marker, has been inserted in place of the deleted sequence.
A deletion that removes part of Rh50. The deleted region is replaced with a 3xP3-DsRed marker and LexA.