FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Clone: Dmel\UT01740
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General Information
Symbol
Dmel\UT01740
Species
D. melanogaster
Name
UT01740
FlyBase ID
FBcl0293050
Feature type
cDNA_clone
Computed gene(s)
Dmel\CG5787
 
Collection Status
N/A
Known Problems
none
FlyBase assessment
    Library
    UT_cDNA
    • UT cDNA library of D. melanogaster, adult male, testis enriched for germ cells by prd overexpression.
    Strain
      Vector
      Tissue Source
      Stage
      Tissue/Position (including subcellular localization)
      Reference
      adult stage | male
      testis
      (Stapleton et al., 2003)
      Sequence Data of the Insert
      3' Sequence
      Total bases
      693
      NCBI
      CK605185
      3' sequence dataSequence Downloader
      ATAGTTATTTCCCAGGGCGCAAGAGACGCCCATCTGGGTAAGTTATTTATTTGTAAAAGCATTGCGACATAATTACAATC
TACTTCTTAAAGTCAAGAGGATGGTTCTTCATGTGTGCAATAATAAAGGTCTCTAATACAACTAGATGCAGACTCATACA
ATCCATAAAATAATGCTGAAGAAGAACCCACGCTCTATTTCCTCCTTTGATGGGTCCAGTGCATATTCGGCTGTTGAGAA
GGAGGGGAGGTGTCTAGTTTCCAAATTACTTTACTCTATAGAGGGAAAGACAGTGCGATGGTCTTTCCATTCGGTTTCGC
TGATTGCCACTAGCTCGTCGAAAGCCTGTTTGGCCAGCCAGTGGAAGCATGGACTCTTCCATTCGCGCGCAATCGCCTGC
TGATCCTTGACGTCGTCCTCGGCGGTGGACTTGGAATTGGTAATGGCCTTGTACTTGGCCCACAGGAAGACCATGCTGCG
CAACTTGTAAACCCACAGGTCATTGGGTATGCCGCGAATAATGTCCTGTATTTCCACCATGTTCAACTCCGTAAGGTTAT
CCAGGAAGGTGCGGTTCATCAACGTAAAGATCGTCTTCATTGTGTCGTACGGCACGTCGGCATTCTTTCTCGAGACAAAG
TGTGCCGACTTTATGATAGCGTACACACCTGCTCTGTGGTTAGCTTGGGCAAT
      5' Sequence
      Total bases
      630
      NCBI
      CK605186
      5' sequence dataSequence Downloader
      CAACAGCTACCCACGTTCCGGAGACGGAGATCGGAGCGGTTTCAATCCGAGCAGCAACCAGCGTTTCGGCGATAATTTCC
AGGACAAATTTCGTGGCGGGCCGTCCTCCAATTTCGGCCAAGCGAACAGGGGAGCATCCAATCAGAGCAACGTAGCCCGC
TTGCTGGAGGTGGAGAACCGTATGGACTGCAATCCAGATGTCAACTATCGAGGTCCCCAGCCGCAGGGAAGCGGCGATAG
GTTTTTCAACGATGAACCCAGAAATCAGGGATCATTCGGTAATTTCGTTCCCAACGATGGAGTGCCATTTGGCGGCAACA
ATGCTCCCTCTTTTGGTGGAAACAATGGACCCGCTTATGGGGGCAATAGTGGACCTTCCTTTGGAGGAAGCGATGTCCCT
TTTGGTGGAAACAGTGGTCCTTCGTTTGGAGGAAACAATCAACGCAACTTCAACAATGACAATTTCCCAAACAGAGGTCC
TAACTATGATGACCAGCGTTCCGGTTTCAATGATTCCTACGGACGCAGTGGAAATCACGGCAACTTTGGCCAGAACTCTG
GAAACTTTGGGGACAACAATAGGGATATAGGAAACTTTAACAACCGCGGACCGGCTGTCGGAGGATCCGG
      Library Information (1)
      Library: UT_cDNA
      Description

      Cloned cDNAs prepared from polyadenylated RNA isolated from adult male testis. The strain used was not the iso-1 sequenced strain.

      (Stapleton et al., 2003)
      Sample preparation

      As a source for stem cell-enriched tissue, testis were dissected from adult males 0-3 days old that overexpressed upd (P{UAS-os.C}) in germ cells from the nos-VP16 GAL4 driver (P{GAL4::VP16-nos.UTR}).

      Protocol

      RNA isolated from dissected testis overexpressing upd to enrich for stem cells was kindly provided by M. Fuller. cDNA directionally cloned directly into EcoRI/XhoI-digested pOTB7 plasmid. cDNAs were transformed into DH5-alpha TonA.

      (Stapleton et al., 2003)
      Comment
      Reported As
      Symbol Synonyms
      UT
      UT_cDNA
      References (1)
      Unpublished (1)
      Stapleton et al., 2003, BDGP/HHMI UT Drosophila EST Project.
      BDGP/HHMI UT Drosophila EST Project. [FBrf0186645]
      External Crossreferences and Linkouts ( 1 )
      Linkouts
      DGRC - Stock center for Drosophila cDNAs, vectors, and cell lines
      Synonyms and Secondary IDs (0)
      Reported As
      Symbol Synonym
      Secondary FlyBase IDs
        References (0)