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General Information
Name
UT_cDNA
Species
D. melanogaster
Reagent type
FlyBase ID
FBlc0000018
Project
Created by
[BDGP: cDNA & EST FAQ](http://www.fruitfly.org/EST/faq.html)
Vector
Title
UT cDNA library of D. melanogaster, adult male, testis enriched for germ cells by prd overexpression.
Accessions
Overview
Description

Cloned cDNAs prepared from polyadenylated RNA isolated from adult male testis. The strain used was not the iso-1 sequenced strain.

Available from
Biosample Source
Overview
Strain
Tissue isolated
Other tissues studied
Cell component
Cell line
Key genes
(overexpressed_factor)
Sample preparation

As a source for stem cell-enriched tissue, testis were dissected from adult males 0-3 days old that overexpressed upd (P{UAS-os.C}) in germ cells from the nos-VP16 GAL4 driver (P{GAL4::VP16-nos.UTR}).

Biosamples used (1)
Biosample
Type
Title
D. melanogaster, adult male, testis enriched for germ cells by prd overexpression, source for cDNA.
Reagent Details
Molecular Construct used
Protocol

RNA isolated from dissected testis overexpressing upd to enrich for stem cells was kindly provided by M. Fuller. cDNA directionally cloned directly into EcoRI/XhoI-digested pOTB7 plasmid. cDNAs were transformed into DH5-alpha TonA.

Mode of Assay
Data analysis
Comments
Associated Data
Size
Associated features
722 Clone(s)
Files
Additional Information
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
UT
UT_cDNA
Name Synonyms
UT cDNA library of D. melanogaster, adult male, testis enriched for germ cells by prd overexpression.
Secondary FlyBase IDs
    References (1)