FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Su(var)205, cav
FlyBase ID
FBig0000095228
Interaction Network
Interactions Browser links
Su(var)205 network
cav network
Reported Interactions
FBrf0137031-1.coIP.WB
Reference
Shareef et al., 2001
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Su(var)205 

HP1

bait
cav 

HOAP

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult heads of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Shareef et al., 2001)
FBrf0137031-2.coIP.WB
Reference
Shareef et al., 2001
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
cav 

HOAP

bait
Su(var)205 

HP1

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult heads of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Shareef et al., 2001)
FBrf0152321-2.Y2H
Reference
Shaffer et al., 2002
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Su(var)205 

HP1

bait

fused to LexA DNA-binding domain

cav 

HOAP

prey

fused to B42 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey from embryo cDNA expression library).

(Shaffer et al., 2002)

Two-hybrid system: yeast LexA-BD/B42-AD

(Shaffer et al., 2002)
FBrf0152321-4.coIP.A
Reference
Shaffer et al., 2002
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Su(var)205 

HP1

bait
cav 

HOAP

prey

[35]S label

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait derived from wild-type embryonic extract; prey synthesized by in vitro translation.

(Shaffer et al., 2002)
FBrf0173167-1.coIP.WB
Reference
Badugu et al., 2003
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Su(var)205 

HP1

bait

FLAG tag

cav 

HOAP

prey

His tag

cav 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
cav 
C-terminal region
sufficient binding region
aa 115-337
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

(Badugu et al., 2003)
FBrf0173167-2.coIP.WB
Reference
Badugu et al., 2003
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
cav 

HOAP

bait

His tag

cav 
cav 
cav 
Su(var)205 

HP1

prey

FLAG tag

Su(var)205 
Su(var)205 
Su(var)205 
Su(var)205 
Su(var)205 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
cav 
C-terminal region
sufficient binding region
aa 115-337
cav 
PETEMNE motif
necessary binding region
PETEMNE

excess PETEMNE peptide blocks hinge binding

cav 
PETEMNE motif
mutation disrupting interaction
aa 290

P290E blocks binding of HP1 hinge domain

Su(var)205 
hinge domain
sufficient binding region
aa 64-149
Su(var)205 
hinge domain
necessary binding region
KSKRTTDAEQDTIPVSGST

peptide blocks binding

Su(var)205 
chromoshadow domain
sufficient binding region
aa 147-206
Su(var)205 
chromoshadow domain
mutation disrupting interaction
aa 190

V190E blocks chromoshadow binding

Su(var)205 
chromoshadow PRMVI motif
necessary binding region
PRMVI

excess PRMVI peptide blocks chromoshadow binding

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

(Badugu et al., 2003)

cav interacts with Su(var)205, but not with HP1b or HP1c.

(Badugu et al., 2003)
FBrf0173167-3.CH.MW
Reference
Badugu et al., 2003
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
cav 

HOAP

neutral component
cav 

HOAP

neutral component
cav 

HOAP

neutral component
Su(var)205 

HP1

neutral component
Su(var)205 

HP1

neutral component
Su(var)205 

HP1

neutral component
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; proteins produced as a recombinant fusion proteins in bacterial system.

(Badugu et al., 2003)
FBrf0173167-7.PD.A
Reference
Badugu et al., 2003
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
cav 

HOAP

bait

His tag

Su(var)205 

HP1

prey

[35]S label

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

cav pulls down Su(var)205, but not HP1b or HP1c.

(Badugu et al., 2003)

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

(Badugu et al., 2003)
FBrf0206682-5.PD.WB
Reference
Raffa et al., 2009
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
Su(var)205 

HP1

bait

GST pull down

cav 

HOAP

prey

FLAG tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Cloned GST-Su(var)205 construct incubated with lysate from S2 cell line transfected with tagged cav construct.

(Raffa et al., 2009)
FBrf0207567-8.PD.WB
Reference
Joppich et al., 2009
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
cav 

HOAP

bait

GST tag

Su(var)205 

HP1

prey

His tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

(Joppich et al., 2009)

Positive control.

(Joppich et al., 2009)
FBrf0210059-1.coIP.MS
Reference
Gao et al., 2010
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage 1embryonic stage 15blastoderm stagegastrula stageembryonic stage 8embryonic stage 9embryonic stage 6early extended germ band stageembryonic cycle 3extended germ band stageembryonic stage 4embryonic stage 2embryonic cycle 9late extended germ band stageembryonic stage 5embryonic stage 12embryonic stage 11embryonic stage 3embryonic cycle 6embryonic cycle 8embryonic cycle 5embryonic cycle 7embryonic stage 13embryonic cycle 4embryonic stage 14embryonic stage 10embryonic stage 7
Cell line used
Participants
Corresponds to
Reported as
Role
Note
cav 

HOAP

bait

Myc tag

Su(var)205 

HP1

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was a chromatin extract from embryos derived from transgenic line carrying tagged cav construct.

(Gao et al., 2010)
FBrf0210059-4.coIP.WB
Reference
Gao et al., 2010
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage 1embryonic stage 15blastoderm stagegastrula stageembryonic stage 8embryonic stage 9embryonic stage 6early extended germ band stageembryonic cycle 3extended germ band stageembryonic stage 4embryonic stage 2embryonic cycle 9late extended germ band stageembryonic stage 5embryonic stage 12embryonic stage 11embryonic stage 3embryonic cycle 6embryonic cycle 8embryonic cycle 5embryonic cycle 7embryonic stage 13embryonic cycle 4embryonic stage 14embryonic stage 10embryonic stage 7
Cell line used
Participants
Corresponds to
Reported as
Role
Note
cav 

HOAP

bait

Myc tag

Su(var)205 

HP1

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was extract from embryos derived from transgenic line carrying tagged cav construct.

(Gao et al., 2010)
FBrf0228809-9.Y2H
Reference
Cenci et al., 2015
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
cav 

HOAP

bait

fused to GAL4 DNA-binding domain

Su(var)205 

HP1

prey

fused to GAL4 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein.

(Cenci et al., 2015)

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Cenci et al., 2015)

Positive control.

(Cenci et al., 2015)
FBrf0230154-2.CH.MW
Reference
Vedelek et al., 2015
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Su(var)205 

HP1

neutral component
Su(var)205 

HP1

neutral component
Su(var)205 

HP1

neutral component
cav 

HOAP

neutral component
cav 

HOAP

neutral component
cav 

HOAP

neutral component
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Su(var)205, cav, moi and ver were co-expressed in bacteria. This was supplemented with purified HipHop, and proteins were separated by heparin-sepharose then size-exclusion chromatography to identify complexes formed.

(Vedelek et al., 2015)

ver-moi and Su(var)205-cav-HipHop form separate subcomplexes in vitro. All five proteins were not observed to form a single "terminin" complex in vitro.

(Vedelek et al., 2015)

Interaction in vitro; proteins produced as a recombinant fusion proteins in bacterial system.

(Vedelek et al., 2015)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (8)