FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
lwr, Sumo
FlyBase ID
FBig0000095415
Interaction Network
Interactions Browser links
lwr network
Sumo network
Reported Interactions
FBrf0123105-1.ENZ.A
Reference
Description
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
lwr 

dUbc9

enzyme

GST tag

lwr 

dUbc9

enzyme

GST tag

Sumo 

dSmt3

enzyme target

GST tag, [32]P label

Sumo 

dSmt3

enzyme target

GST tag, [32]P label

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction
FBrf0132366-10.MW.A
Reference
Long and Griffith, 2000
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
lwr 

DmUbc9

neutral component

GST tag

lwr 

DmUbc9

neutral component

GST tag

lwr 

DmUbc9

neutral component

GST tag

Sumo 

DmSUMO-1

neutral component

GST tag, [32]P label

Sumo 

DmSUMO-1

neutral component

GST tag, [32]P label

Sumo 

DmSUMO-1

neutral component

GST tag, [32]P label

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Purified Aos1 and Uba2 were incubated with smt3 and Ubc9 in the presence of ATP. smt3 was [32]P labeled by phosphorylation of an added protein kinase A site. Conjugation activity was monitored by SDS-PAGE of proteins for the appearance of novel, higher molecular weight proteins.

(Long and Griffith, 2000)

Aos1 and Uba2 transfer activated smt3 (SUMO) to lwr in vitro. This covalent linkage is sensitive to reducing agents (BME), suggesting a thioester bond.

(Long and Griffith, 2000)

Interaction in vitro; proteins produced as a recombinant fusion proteins in bacterial system.

(Long and Griffith, 2000)
FBrf0144953-25.PD.WB
Reference
Shih et al., 2002
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Sumo 

DmSmt3

bait

His tag

Sumo 
lwr 

DmUbc9

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Sumo 
C-terminally cleaved region
sufficient binding region
aa 1-88

coordinates relative to smt3-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from wild-type embryonic extract.

(Shih et al., 2002)
FBrf0218395-10138.DPiM
Reference
Guruharsha et al., 2011
Description
physical association
Assay
Collection
DPiM-2
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
lwr 
unspecified role
lwr 
unspecified role
lwr 
unspecified role
Sumo 
unspecified role
Sumo 
unspecified role
Sumo 
unspecified role
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

HGScore = 61.70737

External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (4)