Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

Weak interaction.

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

The catalytic kinase activity of pll is required for its interaction with dl in this assay.

Two-hybrid system: yeast LexA-BD/B42-AD

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

The catalytic kinase activity of pll is required for its interaction with dl in this assay.

Two-hybrid system: yeast LexA-BD/B42-AD

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey from ovary and embryo cDNA expression library).

Using pll bait in a two hybrid screen of ovary and embryo cDNA libraries, dl represented 39 of 159 hits.

Two-hybrid system: yeast LexA-BD/B42-AD

Interaction in vitro; proteins produced as a recombinant fusion proteins in bacterial system.

Purified pll was assayed for its ability to block the binding of purified dl protein to a [32]P labeled DNA fragment of the zen promoter, as determined by gel shift assays. pll alone did not bind to zen DNA.

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

Two-hybrid system: yeast LexA-BD/B42-AD