ETE substituted with TMT from RRM2 domain, coordinates relative to elav-PA
W419E, coordinates relative to elav-PA
AM substituted with TR from RRM2 domain, coordinates relative to elav-PA
Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).
Two-hybrid system: yeast GAL4-BD/GAL4-AD
Source was adults of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.
RNA immunoprecipitated from formaldehyde fixed embryos.
elav protein binds transcripts with developmentally regulated 3\' UTR extensions.
Source was embryonic nuclear extract of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.
Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.
Positive control.
