Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

Deletion of the drl WIF domain bypasses the requirement of Wnt5 for drl homodimerization.

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

drl homodimers are present at the cell surface, as indicated by biotinylation of co-immunoprecipitated FLAG-tagged drl protein.

Cells were treated with a membrane-impermeable cell surface biotinylation reagent before lysis and anti-HA immunoprecipitation.

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

The WIF (Wnt inhibitory factor), extracellular TBC (tetrabasic cleavage) and intracellular domains are dispensable for drl homodimerization.

Source was bacterial cell line; protein produced as transgenic fusion protein.

The drl transmembrane domain was cloned into the pccKAN vector, fusing it to an extracellular MBP domain (periplasmic localization) and an intracellular ToxR cytoplasmic domain. Upon expression in E. coli, dimerization of the ToxR domain at the inner membrane results in transcriptional activation of a CAT reporter under control of the ctx promoter.