FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
blow, Vrp1
FlyBase ID
FBig0000102740
Interaction Network
Interactions Browser links
blow network
Vrp1 network
Reported Interactions
FBrf0213682-2.coIP.WB
Reference
Jin et al., 2011
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2R+
Participants
Corresponds to
Reported as
Role
Note
blow 

Blow

bait

V5 tag

blow 
blow 
Vrp1 

Sltr

prey

FLAG tag

Vrp1 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
blow 
N terminal region
necessary binding region
aa 1-99
blow 
C terminal region
necessary binding region
aa 472-644
Vrp1 
WASP-binding domain
necessary binding region
aa 683-706

deletion of an internal 24 amino acid sequence including the WASP-binding domain

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2R+ cell line; bait produced from transfected construct; prey produced from transfected construct.

(Jin et al., 2011)

blow Y378F binds to Vrp1 but not Crk.

(Jin et al., 2011)

WASp and blow bind competitively to Vrp1. Vrp1 binds to blow with 7-fold lower affinity than it binds WASp.

(Jin et al., 2011)
FBrf0213682-4.coIP.WB
Reference
Jin et al., 2011
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2R+
Participants
Corresponds to
Reported as
Role
Note
blow 

Blow

bait

V5 tag

Vrp1 

Sltr

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2R+ cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Jin et al., 2011)

Authors overexpressed blow in S2R+ cells and co-precipitated endogenous Vrp1.

(Jin et al., 2011)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)