FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Rbp4, fest, Polr2D
FlyBase ID
FBig0000103755
Interaction Network
Interactions Browser links
Rbp4 network
fest network
Polr2D network
Reported Interactions
FBrf0229780-1.coIP.WB
Reference
Baker et al., 2015
Description
physical association
Assay
Collection
Source/Stage
testis / adult stagemale
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Rbp4 

Rbp4

bait

eYFP tag

fest 

Fest

prey

HA tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult male testis of transgenic fly line; bait produced from tagged transgenic construct; prey produced from tagged transgenic construct.

(Baker et al., 2015)
FBrf0229780-2.coIP.WB
Reference
Baker et al., 2015
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
fest 

Fest

bait

HA tag

Rbp4 

Rbp4

prey

Myc tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Baker et al., 2015)
FBrf0229780-3.coIP.WB
Reference
Baker et al., 2015
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
Rbp4 

Rbp4

bait

Myc tag

fest 

Fest

prey

HA tag

Polr2D 
Polr2D 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Polr2D 
N-terminal region
sufficient binding region
aa 1-275
Polr2D 
N-terminal region
necessary binding region
aa 245-275
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Baker et al., 2015)
FBrf0258157-3.coIP.WB
Reference
Baker et al., 2023
Description
physical association
Assay
Collection
Source/Stage
testis / maleadult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
fest 

fest

bait

YFP tag

Rbp4 

Rbp4

prey

HA tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult testes of transgenic fly line; bait and prey produced from tagged transgenic constructs.

(Baker et al., 2023)
FBrf0258157-6.coIP.MS
Reference
Baker et al., 2023
Description
physical association
Assay
Collection
Source/Stage
testis / maleadult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Rbp4 

Rbp4

bait

YFP tag

fest 

fest

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult testes of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Baker et al., 2023)
FBrf0258157-7.coIP.MS
Reference
Baker et al., 2023
Description
physical association
Assay
Collection
Source/Stage
testis / maleadult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
fest 

fest

bait

YFP tag

Rbp4 

Rbp4

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult testes of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Baker et al., 2023)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)