Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

Expression of the constitutively active sev[S11] construct leads to tyrosine phosphorylation of Dab.

The sev S11 constitutively active construct lacks most of the extracellular domain, fused to the Lcp3 signal peptide.

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from transfected S2 cell extract.

Direct binding of Dab to the sev C-terminus could be be tested due to poor solubility of the sev peptides.