FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
trn, Ptp52F
FlyBase ID
FBig0000110701
Interaction Network
Interactions Browser links
trn network
Ptp52F network
Reported Interactions
FBrf0215392-10.coIP.WB
Reference
Bugga et al., 2009
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
trn 

trn

bait

GFP tag

Ptp52F 

Ptp52F

prey

MYC tag

Ptp52F 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Ptp52F 
phosphatase domain
mutation increasing interaction
aa 1258

D1258A, D to A substrate trapping mutation.

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Bugga et al., 2009)
FBrf0215392-11.PD.WB
Reference
Bugga et al., 2009
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
Ptp52F 

Ptp52F

bait

GST tag

trn 

trn

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Bugga et al., 2009)
FBrf0215392-3.Y2H
Reference
Bugga et al., 2009
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Ptp52F 

Ptp52F

bait

fused to LexA DNA-binding domain

Ptp52F 
trn 

trn

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Ptp52F 
phosphatase domain
mutation increasing interaction
aa 1258

D1258A, D to A substrate trapping mutation.

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey from embryo cDNA expression library).

(Bugga et al., 2009)

Two-hybrid system: yeast LexA-BD

(Bugga et al., 2009)
FBrf0215392-9.coIP.WB
Reference
Bugga et al., 2009
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
Ptp52F 

Ptp52F

bait

MYC tag

Ptp52F 
trn 

trn

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Ptp52F 
phosphatase domain
mutation increasing interaction
aa 1258

D1258A, D to A substrate trapping mutation.

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Bugga et al., 2009)
External Crossreferences and Linkouts ( 0 )
References (1)