XYY males were used as progenitors. These males carried two Y chromosomes: Dp(1;Y)B^S (dominant marker Bar-Stone on the tip of Y's long arm) and Dp(1;Y)y⁺ (insertional duplication of X, including the wild-type allele of yellow, near the tip of Y's long arm). These males were X-irradiated and crossed to homozygous X-balancer females. Single F1 females (each of which carried one of the parental Y chromosomes) were crossed to males carrying the other marked Y chromosome; transmission through the female allowed recovery of lethal or sterile events. The X;Y translocations were recognized by the occurrence of linkage between the maternally derived X and Y chromosomes in the F2. 87 lines, 22 marked with y[+] and 65 marked with B[S], were established and characterized. Of these, 17 were T(X;Y;A) translocations, including one that did not appear to involve a direct T(X;Y) and thus was eliminated from further analysis.