FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Assay: RNA-Seq_GSE38764_L3_CNS_neuroblast
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General Information
Name
RNA-Seq_GSE38764_L3_CNS_neuroblast
Species
D. melanogaster
Assay type
RNA-Seq
FlyBase ID
FBlc0001035
Project
Data Provider
Title
RNA-Seq of D. melanogaster, larval neuroblast.
Accessions
Biosample Source
Overview
Strain
Stage
larval stage
(Berger et al., 2012)
Sex
Tissue isolated
larval neuroblast
(Berger et al., 2012)
larval central nervous system
(Berger et al., 2012)
Other tissues studied
Cell component
Cell line
Key genes
Methods
targeted cell labeling
(Berger et al., 2012)
Sample preparation

Neural cells were fluorescently labeled using GFP under the control of ase regulatory sequences and FACS-sorted into neuroblasts and neurons. Neuroblasts were distinguished by their strong GFP signal, large size and relatively small population, while neurons were identified by their weaker GFP signal, smaller size and greater abundance.

Biosamples used (1)
Biosample
Type
Title
BS_Knoblich_L3_CNS_neuroblast
isolated cells
D. melanogaster, larval neuroblast, source for RNA.
Assay Details
Methods
poly(A) RNA isolation
(Berger et al., 2012)
paired-end layout
(Berger et al., 2012)
Illumina sequencing
(Berger et al., 2012)
Key genes
Protocol

Poly(A)+ RNA was subjected to first strand cDNA synthesis. Second strand cDNA synthesis was done in the presence of UTP to generate stranded cDNA libraries. Double-stranded cDNA was end-repaired and ligated to adapters. After size selection (200-600bp) and UDG-ase treatment for strand specificity, DNA was PCR amplified and the quality assessed by Agilent bioanalyzer.

Total RNA was isolated from 200,000-250,000 FACS-sorted neuroblasts using Trizol. Polyadenylated mRNA was enriched for using oligo(dT) beads. The poly(A)+ RNA was fragmented using divalent cations under elevated temperature.

(Berger et al., 2012)
Mode of Assay

Read length (bases):76

(Berger et al., 2012)
Comments
Associated Data
Size
Files
Additional Information
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
RNA-Seq_GSE38764_L3_CNS_neuroblast
Name Synonyms
RNA-Seq of D. melanogaster, larval neuroblast.
(Berger et al., 2012)
Secondary FlyBase IDs
    References (2)