Abstract
Anti-Drosophila chromatin remodeling protein 1 (CRPl) and anti-Drosophila DNA topoisomerase II (topo II) polyclonal antibodies were affinity purified and used with antihistone monoclonal antibodies to conduct confocal laser scanning immunofluorescence microscopy in Drosophila adult male accessory gland and third instar larva salivary gland nuclei. Except for the nucleolus, topo II and histones were distributed throughout interphase nuclei, whereas, CRPl was diffusely distributed throughout the nuclear interior, including the nucleolus. CRPl also showed areas of colocalization with both histones (chromatin) and topo II (karyoskeleton). The above observations were made under reduced background fluorescence and fluorochrome photobleaching conditions and support the notion that CRPl constitutes a nuclear domain that shares space with but is distinct from, both the chromatin and the karyoskeleton. (The J Histotechnol 22:23, 1999)
