Gene or accession: CG7981
Gene annotation error
Genes CG7981 and CG12497 should be merged.
Comments: We think CG7981 and CG12497 are parts of the same gene for the
following reasons:
1) starting observation: we have analysed a series of protein-trap lines
showing basement membrane localization (the normal localization of
CG7981/Trol/perlecan). Sequence of the insertion site of these lines indicate
that they are inserted upstream of the current 5' most exon of CG7981 (around
position 159000 of AE003424). This suggested to us that the annotation for
CG7981 (a huge gene) could be only partially accurate.
Looking at the genome annotation, we found that:
2) the 5' most EST (HL01107) used for annotation of CG7981 seem to be non
coding since several STOP codons are found in the 3 frames.
3) similarly, there are currently no EST to confirm the structure of gene
CG12497 (model mRNA has currently no 5' and 3' UTR).
4) CG12497 encodes for 2 LDL domains, which are found in great number in
CG7981 N-terminus.
For these reasons, we believe that a new model based on the merge of CG12497
and CG7981 for the Trol/perlecan gene should be tested.
Looking forward to hear from you.
Thanks.
Stephane