Isolation and characterization of Df(2L)BSC192
Stacey Christensen and Kevin Cook
Bloomington Stock Center
Indiana University
Df(2L)BSC192 was isolated as a FLP recombinase-induced recombination event involving P{XP}TepIIId03976 and PBac{WH}CG7221f04545. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of P{hsFLP}1, y1 w1118; P{XP}TepIIId03976/PBac{WH}CG7221f04545 males crossed to w1118; P{hs-hid}2, wgSp-1/CyO females. These males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP5.WH5}BSC192 from the segment of P{XP}TepIIId03976 to the left of its FRT site and the segment of PBac{WH}CG7221f04545 to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. The cytological breakpoints of Df(2L)BSC192 predicted from the Release 4 genomic coordinates of the transposable element insertion sites are 28C1;28D3. It failed to complement mtsXE-2258 and Df(2L)BSC142.