Isolation and characterization of Df(2L)BSC190
Stacey Christensen and Kevin Cook
Bloomington Stock Center
Indiana University
Df(2L)BSC190 was isolated as a FLP recombinase-induced recombination event involving PBac{WH}CG5973f03909 and P{XP}d05214. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of P{hsFLP}1, y1 w1118; PBac{WH}CG5973f03909/P{XP}d05214 males crossed to w1118; P{hs-hid}2, wgSp-1/CyO females. These males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP5.WH5}BSC190 from the segment of PBac{WH}CG5973f03909 to the left of its FRT site and the segment of P{XP}d05214 to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. The cytological breakpoints of Df(2L)BSC190 predicted from the Release 4 genomic coordinates of the transposable element insertion sites are 27F6;28D2. It failed to complement mtsXE-2258 and Df(2L)ED499.