Isolation and characterization of Df(2L)BSC216
Stacey Christensen and Kevin Cook
Bloomington Stock Center
Indiana University
Df(2L)BSC216 was isolated as a FLP recombinase-induced recombination event involving PBac{WH}Nckx30Cf04751 and P{XP}CG13124d10773. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of P{hsFLP}1, y1 w1118; PBac{WH}Nckx30Cf04751/P{XP}CG13124d10773 males crossed to w1118; P{hs-hid}2, wgSp-1/CyO females. These males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP5.WH5}BSC216 from the segment of PBac{WH}Nckx30Cf04751 to the left of its FRT site and the segment of P{XP}CG13124d10773 to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. Exelixis, Inc. determined the insertion site of P{XP}CG13124d10773 to be at Release 3 genomic coordinate 9900868, which is predicted to lie in 30E3 on the Release 3 map and 30E1 on the Release 4 map. PBac{WH}Nckx30Cf04751 is predicted to lie in 30C6 on the Release 4 map. Consequently, the cytological breakpoints of Df(2L)BSC216 are predicted to be 30C6;30E1. It failed to complement undDelta34.