Isolation and characterization of Df(1)BSC352
Stacey Christensen and Kevin Cook
Bloomington Stock Center
Indiana University
Df(1)BSC352 was isolated as a FLP recombinase-induced recombination event involving PBac{WH}f06492 and P{XP}CrebB-17Ad01424. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of PBac{WH}f06492/P{XP}CrebB-17Ad01424; MKRS, P{hsFLP}86E/+ females crossed to Binsinscy/Y males. These females were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP5.WH5}BSC352 from the segment of PBac{WH}f06492 to the left of its FRT site and the segment of P{XP}CrebB-17Ad01424 to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. Exelixis, Inc. determined the insertion site of PBac{WH}f06492 to be at Release 3 genomic coordinate 17848426 on chromosome 1. This corresponds to 16F7 on both the Release 3 and 5 genome maps. The predicted position of P{XP}CrebB-17Ad01424 on the Release 5 map is 17A8. Consequently, the cytological breakpoints of Df(1)BSC352 are predicted to be 16F7;17A8. It failed to complement oso. Df(1)FDD-0383177 is a synonym for Df(1)BSC352.