Isolation and characterization of Df(3R)BSC317
Stacey Christensen, Jill Gresens, Kimberley Cook and Kevin Cook
Bloomington Stock Center
Indiana University
Df(3R)BSC317 was isolated as a FLP recombinase-induced recombination event involving PBac{WH}twinf05399 and P{XP}crbd02006. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of w1118; Dr1/TM6C, Sb1 females crossed to P{hsFLP}1, y1 w1118; PBac{WH}twinf05399/P{XP}crbd02006 males. These males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP5.WH5}BSC317 from the segment of PBac{WH}twinf05399 to the left of its FRT site and the segment of P{XP}crbd02006 to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. The cytological breakpoints of Df(3R)BSC317 predicted from the Release 5 genomic coordinates of the transposable element insertion sites are 95F2;95F11. Df(3R)BSC317 failed to complement jar322 and jar2095. Df(3R)FDD-0359031 is a synonym for Df(3R)BSC317.