Isolation and characterization of Df(3R)BSC808
Stacey Christensen, Kim Cook and Kevin Cook
Bloomington Stock Center
Indiana University
Df(3R)BSC808 was isolated as a FLP recombinase-induced recombination event involving PBac{WH}CG6231f01139 and P{XP}d05044. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of w1118; P{hs-hid}3, Dr1/TM6C, Sb1 cu1 females crossed to P{hsFLP}1, y1 w1118; PBac{WH}CG6231f01139/P{XP}d05044 males. The males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP5.WH5}BSC808 from the segment of PBac{WH}CG6231f01139 to the left of its FRT site and the segment of P{XP}d05044 to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. Exelixis, Inc. determined the insertion site of P{XP}d05044 to be Release 3 genomic coordinate 16110153 on chromosome arm 3R. This corresponds to Release 5 coordinate  3R:16110136 . The insertion site of PBac{WH}CG6231f01139 is Release 5 coordinate  3R:15444823 . Consequently, the breakpoints of Df(3R)BSC808 predicted from the Release 5 genomic coordinates of the transposable element insertion sites are  3R:15444823 ; 3R:16110136  and the cytological breakpoints predicted from these coordinates are 92A11;92E1. Df(3R)BSC808 failed to complement bnl00857 and Vha1305113.