Mutations and chromosomal aberrations in the region of Tis11 and Smr
Robert Fedic*, Youn-Jeong Choi*, Eric Spana#, Kevin Cook^ and Jim Mason*
*National Institute of Environmental Health Sciences
#Duke University
^Bloomington Stock Center, Indiana University
P{EP}Tis11G0147 was generated by GenExel, Inc.  It is inserted immediately downstream of the Tis11 (FBgn0011837) promoter.  Df(1)IE35 is an imprecise excision of P{EP}Tis11G0147 that extends proximally 200 kb. (Tis11IE35 is associated with the distal Df(1)IE35 breakpoint.) The lethality of Df(1)IE35 is rescued by Dp(1;Y)BSC5 (FBab0029206), which carries Smr (FBgn0263865), but not Tis11. Df(1)IE35/Dp(1;Y)BSC5 males are completely deficient for Tis11, but are viable and fertile.
This data indicates that the distal Dp(1;Y)BSC5 breakpoint lies somewhere between the distal end of Tis11 (11B9,  X:12553395  (R5)) and the distal end of Smr (11B10,  X:12578236  (R5)).  This is consistent with Comparative Genomic Hybridization (CGH) microarray data placing the breakpoint between sequences in CkIalpha (11B7,  X:12548008..12548076  (R5)) and CG15725 (11B12,  X:12602220..12602288  (R5)).
The proximal Dp(1;Y)BSC5 breakpoint lies within CG12716 (FBgn0030439) or CG32643 (FBgn0052643) or in the region between these genes in the range 11D5-6,  X:12869529..12878892  (R5) based on CGH microarray data showing the duplication of sequences within CG12716 ( X:12869470..12869529  (R5)), but no duplication of sequences within CG32643 ( X:12878892..12878960  (R5)).
The rest of the microarray data are consistent with genes between CG15725 and CG12716 being duplicated in Dp(1;Y)BSC5.