After generating all the Dp(1;Y)BSC chromosomes as described in FBrf0212582, we calculated the minimal euchromatic coverage of the X chromosome provided by these duplications as 97.0%.
Series of Dp(1;Y) stocks carrying segments of the X chromosome on the tip of Y chromosome; in an isogenic background. At the time of publication (FBrf0212582), the Dp(1;Y) collection provided a minimum of 78% coverage with duplication breakpoints spaced a median of nine genes apart.
11B17;h26-h32
The lethality of Df(1)IE35 is rescued by Dp(1;Y)BSC5.
Male fertile.
The complementation of Df(1)IE35 by Dp(1;Y)BSC5 indicates that the distal breakpoint of Dp(1;Y)BSC5 lies between the distal end of Tis11 (11B9, X:12553395 (R5)) and the distal end of Smr (11B10, X:12578236 (R5)). This is consistent with Comparative Genomic Hybridization (CGH) microarray data which places the breakpoint between sequences in CkIα (11B7, X:12548008..12548076 (R5)) and CG15725 (11B12, X:12602220..12602288 (R5)).
The proximal Dp(1;Y)BSC5 breakpoint lies within CG12716 (FBgn0030439) or CG32643 (FBgn0052643) or in the region between these genes in the range 11D5-6, X:12869529..12878892 (R5) based on CGH microarray data showing the duplication of sequences within CG12716 ( X:12869470..12869529 (R5)), but no duplication of sequences within CG32643 ( X:12878892..12878960 (R5)).
The cytologically observed breakpoints may not represent the full extent of the duplicated segment, because euchromatic bands juxtaposed to centric heterochromatin may not be visible.
The polytene bands closest to the chromocenter in squashes appear to be from region 11.