cbtD148 is a simple short deletion, while cbtD41 has 11 bases (GATGAAATAAT) inserted (or remaining from the P element) at the excision site.
Based on our mapping results, cbtD41 did not impair the first intron but deleted 241nt of the second intron and caused a frameshift of all three isoforms (cbt-RA, cbt-RB, and cbt-RC). cbtD148 deleted 59nt of the first intron of cbt-RA but did not impinge on cbt-RB and cbt-RC. Following genetic complementation tests indicated that cbtD41 is a null allele, but cbtD148 is not.
The associated file contains the detailed mapping strategy and sequencing results of both alleles, cbtD41 and cbtD148.