The following information accompanied stocks donated to the Bloomington Drosophila Stock Center by Alex Whitworth, University of Cambridge.
TI{TI}Mrm2PRG is an insertion of pHD-DsRed-attP between 3R:19861870_19861877 (r6.45), made via CRISPR/Cas9 and HDR-mediated knock-in using gRNA targeting 3R:19861870-19861871 . The integration was confirmed by PCR. TI{TI}MRM2PRG is homozygous lethal, recapitulating the phenotype observed upon silencing of this gene (see FBrf0252739).