FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
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Luo, L. (2022.10.21). Conditionally-tagged genes from Liqun Luo. 
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FBrf0254816
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Personal communication to FlyBase
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Text of Personal Communication 
The following information accompanied stocks donated to the Bloomington Drosophila Stock Center by Liqun Luo, Stanford University.
A CRISPR-based strategy was used to put a “FRT-1xV5-6xStop-loxP-mini-White-loxP-FRT-3xFLAG-6xStop” cassette in place of the stop codon in the indicated gene, followed by the removal of the loxP-flanked mini-w+, to make conditionally-tagged proteins. In the absence of FLP recombinase, the protein is tagged with V5. In the presence of FLP recombinase, the V5 tag is removed and the protein is tagged instead with FLAG. See Li et al., 2020 (FBrf0244620) for details.
TI{TI}dally<up> Tag:V5.FRT </up>
TI{TI}dpr15<up> Tag:V5.FRT </up>
TI{TI}Hasp<up> Tag:V5.FRT </up>
TI{TI}mmd<up> Tag:V5.FRT </up>
TI{TI}otk<up>RB. Tag:V5.FRT </up> – The cassette was used to replace the stop codon for the RB transcript and should tag otk-PB.
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Research paper

Cell-Surface Proteomic Profiling in the Fly Brain Uncovers Wiring Regulators.
Li et al., 2020, Cell 180(2): 373--386.e15 [FBrf0244620]

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