Tong, Y., Wei, Y., Ju, Y., Li, P., Zhang, Y., Li, L., Gao, L., Liu, S., Liu, D., Hu, Y., Li, Z., Yu, H., Luo, Y., Wang, J., Wang, Y., Zhang, Y. (2023). Anaerobic purinolytic enzymes enable dietary purine clearance by engineered gut bacteria.  Cell Chem. Biol. 30(9): 1104--1114.e7.

FBrf0257638

Research paper

Uric acid, the end product of purine degradation, causes hyperuricemia and gout, afflicting hundreds of millions of people. The debilitating effects of gout are exacerbated by dietary purine intake, and thus a potential therapeutic strategy is to enhance purine degradation in the gut microbiome. Aerobic purine degradation involves oxidative dearomatization of uric acid catalyzed by the O2-dependent uricase. The enzymes involved in purine degradation in strictly anaerobic bacteria remain unknown. Here we report the identification and characterization of these enzymes, which include four hydrolases belonging to different enzyme families, and a prenyl-flavin mononucleotide-dependent decarboxylase. Introduction of the first two hydrolases to Escherichia coli Nissle 1917 enabled its anaerobic growth on xanthine as the sole nitrogen source. Oral supplementation of these engineered probiotics ameliorated hyperuricemia in a Drosophila melanogaster model, including the formation of renal uric acid stones and a shortened lifespan, providing a route toward the development of purinolytic probiotics.