FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Insertion: Dmel\PBac{GAL4D,EYFP}GlcAT-PPL00294
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General Information
Symbol
Dmel\PBac{GAL4D,EYFP}GlcAT-PPL00294
Species
D. melanogaster
Name
FlyBase ID
FBti0058247
Feature type
transposable_element_insertion_site
Description
Inserted Element
PBac{GAL4D,EYFP}
LINE ID

PL00294-5prime

PL00294

Affected gene(s)
GlcAT-P, Scer\GAL4
Viability / fertility
fertile, viable
Associated allele(s)
GlcAT-PPL00294, Scer\GAL4PL00294
Stock availability
1 publicly available
Genomic Location
Chromosomal Location
3L ( 68B1 )
Sequence Location
3L:11,185,566..11,185,566 [-]
Target / Docking site
Genomic Maps
Member of Large Scale Dataset(s)
Dataset
TI_set_PBac{GAL4D,EYFP}.PL
Description

Insertion lines from this collection were assessed for inclusion in the Gene Disruption Project collection; some lines were mapped by the GDP.

(Bellen et al., 2004)

A set of transgenic insertion stocks derived by TE mobilization using the "piggyBac mutator" construct PBac{GAL4D,EYFP}. The dataset consists primarily of insertions on the third chromosomes that also carry proximal Scer\FRT sites, to allow generation of germ-line clones. The PBac{GAL4D,EYFP} construct carries the yellow fluorescent marker Avic\GFPEYFP.3xP3 (which can be distinguished from other GFP-based markers) and a modified Scer\GAL4 driver/reporter gene.

(Hacker et al., 2003)
Detailed Mapping Data
Chromosome (arm)
Sequence Location
3L:11,185,566..11,185,566 [-]
(FlyBase, 2007.6)
Orientation
Cytological location (computed by FlyBase)
68B1 (inferred by FlyBase from sequence location)
Cytological location (reported)
68B1 (reported as inferred from sequence location)
(Häcker et al., 2004)
Insertion into Natural transposon
Comments concerning location
Sequence Data
Inserted Element
Construct
PBac{GAL4D,EYFP}
(FlyBase, 2007.6)
Size (Kb)
Component Allele(s)
Molecular map
Affected Gene(s)
Insertion may affect gene
GlcAT-P
(Pandey et al., 2011, Häcker et al., 2004)
Scer\GAL4
(Pandey et al., 2011)
Variant Molecular Consequences
Phenotypic Data
Expression Data
Reporter Expression
distribution deduced from reporter (Gal4 UAS)
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage 14
embryonic/larval salivary gland
(Pandey et al., 2011)
amnioserosa
(Pandey et al., 2011)
embryonic stage 17
embryonic/larval gut
(Pandey et al., 2011)
third instar larval stage
larval tracheal system
(Pandey et al., 2011)
larval glial cell | subset
(Pandey et al., 2011)
larval neuron | subset
(Pandey et al., 2011)
Kenyon cell
(Pandey et al., 2011)
mushroom body
(Pandey et al., 2011)
larval VUM motor neuron
(Pandey et al., 2011)
Additional Information
Statement
Reference

Reporter expression is observed in embryos in the salivary gland, amnioserosa, and gut, recapitulating aspects of the endogenous in situ pattern of GlcAT-P. In larvae, expression is observed in the tracheal system, in a subset of neurons and glia in the larval brain and ventral nerve cord, as well as in Kenyon cells. Expression is observed two ventral nerve cord neurons per neuromere that were identified as VUM motorneurons.

(Pandey et al., 2011)
 
Marker for
Reflects expression of
GlcAT-P
(Pandey et al., 2011)
Reporter construct used in assay
Mmus\Cd8aUAS.mCherry
(Pandey et al., 2011)
Data on Genetic Line
Line ID

PL00294-5prime

(FlyBase, 2007.6)

PL00294

(Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
Progenitor(s) within the Genome
Related Aberration or Balancer
Aberration
Balancer
Stocks (1)
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
GlcAT-P-Gal4
(Pandey et al., 2011)
PBac{GAL4D,EYFP}GlcAT-PPL00294
(Pandey et al., 2011, FlyBase, 1992-)
Name Synonyms
Secondary FlyBase IDs
    References (8)