phiC31-mediated recombination has been used to replace the attP cassette of the Mi{MIC} element with the intron phase 2 "Trojan GAL4" cassette from the pBS-KS-attB2-SA(2)-T2A-Gal4-Hsp70 plasmid.
FlyBase curator comment: the 5' and 3' flanking sequences obtained for the original MI03631 line map 1bp apart on opposite strands. This may well reflect either a sequencing artifact or chromosomal rearrangement, but at present the line is annotated in FlyBase as having two separate insertions to cope with this discrepancy. Since the Mi{Trojan-GAL4.2}GluRIIBMI03631-TG4.2 Trojan-MiMIC replacement line shows Scer\GAL4 expression in the pattern of GluRIIB, it has been assumed that the insertion annotated in the correct orientation for gene trapping (Mi{MIC}GluRIIBMI03631a) was the substrate for recombination in the event that generated the Mi{Trojan-GAL4.2}GluRIIBMI03631-TG4.2 Trojan-MiMIC replacement line.
phiC31-mediated recombination has been used to replace the attP cassette of the Mi{MIC} element with the intron phase 2 "Trojan GAL4" cassette from the pBS-KS-attB2-SA(2)-T2A-Gal4-Hsp70 plasmid.
FlyBase curator comment: the 5' and 3' flanking sequences obtained for the original MI03631 line map 1bp apart on opposite strands. This may well reflect either a sequencing artifact or chromosomal rearrangement, but at present the line is annotated in FlyBase as having two separate insertions to cope with this discrepancy. Since the Mi{Trojan-GAL4.2}GluRIIBMI03631-TG4.2 Trojan-MiMIC replacement line shows Scer\GAL4 expression in the pattern of GluRIIB, it has been assumed that the insertion annotated in the correct orientation for gene trapping (Mi{MIC}GluRIIBMI03631a) was the substrate for recombination in the event that generated the Mi{Trojan-GAL4.2}GluRIIBMI03631-TG4.2 Trojan-MiMIC replacement line.