FlyBase Gene Report: Dmel\GluRIIB

General Information

Symbol

Dmel\GluRIIB

Species

D. melanogaster

Name

Glutamate receptor IIB

Annotation Symbol

CG7234

Feature Type

protein_coding_gene

FlyBase ID

FBgn0020429

Gene Model Status

Current

Stock Availability

11 publicly available

Gene Summary

Glutamate receptor IIB (GluRIIB) encodes a subunit of the muscle glutamate receptor that responds to neurotransmitter at the neuromuscular junction. [Date last reviewed: 2019-09-12] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
Gene Group
Interactive Fly

Also Known As

DGluRIIB

Key Links

Genomic Location

Cytogenetic map

25E6-25E6

Sequence location

2L:5,559,342..5,563,502 [+]

Recombination map

2-17

RefSeq locus

NT_033779 REGION:5559342..5563502

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (21 terms)

Molecular Function (8 terms)

Terms Based on Experimental Evidence (2 terms)

CV Term

Evidence

References

enables glutamate-gated calcium ion channel activity

inferred from direct assay

(Han et al., 2015)

enables glutamate-gated receptor activity

inferred from mutant phenotype

(Qin et al., 2005)

Terms Based on Predictions or Assertions (6 terms)

CV Term

Evidence

References

enables glutamate receptor activity

inferred from biological aspect of ancestor with PANTHER:PTN001826301

(GO Reference Genome Project, 2011-)

enables kainate selective glutamate receptor activity

inferred from sequence or structural similarity with UniProtKB:P39086

(Gene Ontology Curators, 2002-)

inferred from sequence or structural similarity

(Littleton and Ganetzky, 2000)

enables ligand-gated monoatomic ion channel activity

inferred from electronic annotation with InterPro:IPR001320, InterPro:IPR019594

(InterPro Project Members, 2004-)

enables monoatomic ion channel activity

inferred from electronic annotation with InterPro:IPR001508

(InterPro Project Members, 2004-)

enables signaling receptor activity

inferred from electronic annotation with InterPro:IPR001508

(InterPro Project Members, 2004-)

enables transmitter-gated monoatomic ion channel activity involved in regulation of postsynaptic membrane potential

inferred from biological aspect of ancestor with PANTHER:PTN001826301

(GO Reference Genome Project, 2011-)

Biological Process (6 terms)

Terms Based on Experimental Evidence (3 terms)

CV Term

Evidence

References

involved_in calcium ion transport

inferred from direct assay

(Han et al., 2015)

involved_in neuromuscular synaptic transmission

inferred from mutant phenotype

(Qin et al., 2005)

involved_in synaptic transmission, glutamatergic

inferred from mutant phenotype

(Qin et al., 2005)

Terms Based on Predictions or Assertions (4 terms)

CV Term

Evidence

References

involved_in modulation of chemical synaptic transmission

inferred from biological aspect of ancestor with PANTHER:PTN001826301

(GO Reference Genome Project, 2011-)

involved_in monoatomic ion transport

inferred from electronic annotation with InterPro:IPR001508

(InterPro Project Members, 2004-)

involved_in regulation of synaptic activity

inferred from sequence or structural similarity with FLYBASE:GluRIIA; FB:FBgn0004620

(Gene Ontology Curators, 2002-)

involved_in synaptic transmission, glutamatergic

inferred from biological aspect of ancestor with PANTHER:PTN001826301

(GO Reference Genome Project, 2011-)

Cellular Component (7 terms)

Terms Based on Experimental Evidence (4 terms)

CV Term

Evidence

References

part_of ionotropic glutamate receptor complex

inferred from mutant phenotype

(Qin et al., 2005)

located_in muscle cell postsynaptic specialization

inferred from direct assay

(Qin et al., 2005)

located_in neuromuscular junction

inferred from direct assay

(Jantrapirom et al., 2019, Karr et al., 2009, Kohsaka et al., 2007, Petersen et al., 1997)

located_in postsynaptic membrane

inferred from direct assay

(Kohsaka et al., 2007)

Terms Based on Predictions or Assertions (3 terms)

CV Term

Evidence

References

located_in membrane

inferred from electronic annotation with InterPro:IPR001320, InterPro:IPR001508, InterPro:IPR019594

(InterPro Project Members, 2004-)

is_active_in plasma membrane

inferred from biological aspect of ancestor with PANTHER:PTN000437926

(GO Reference Genome Project, 2011-)

located_in plasma membrane

inferred from sequence or structural similarity with FLYBASE:GluRIIA; FB:FBgn0004620

(Petersen et al., 1997)

is_active_in postsynaptic density membrane

inferred from biological aspect of ancestor with PANTHER:PTN001826301

(GO Reference Genome Project, 2011-)

Gene Group (FlyBase)

NON-NMDA IONOTROPIC GLUTAMATE RECEPTORS - GLURII NEUROMUSCULAR JUNCTION-SUBTYPE

Protein Family (UniProt)

Protein Signatures (InterPro)

Summaries

Gene Snapshot

Glutamate receptor IIB (GluRIIB) encodes a subunit of the muscle glutamate receptor that responds to neurotransmitter at the neuromuscular junction. [Date last reviewed: 2019-09-12]

Gene Group (FlyBase)

NON-NMDA IONOTROPIC GLUTAMATE RECEPTORS - GLURII NEUROMUSCULAR JUNCTION-SUBTYPE -: In Drosophila, neuromuscular junction type A and type B glutamate receptors are heterotetramers assembled from either GluRIIA or GluRIIB plus other GluRII subunits. In response to the neurotransmitter, glutamate, they conduct Ca2⁺. (Adapted from FBrf0228432).

Summary (Interactive Fly)

transmembrane Ca2+ channel protein - expressed on muscular synaptic boutons targeted by motor neurons

Interactive Fly

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\GluRIIB for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry Q9VMP3)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

PDB - An information portal to biological macromolecular structures

4WXJ

Comments on Gene Model

Gene model reviewed during 5.52

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

GluRIIB-RI

FBtr0089577

NM_078761

2949

913

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

GluRIIB-PI

FBpp0088541

104.3

913

6.38

Q9VMP3

NP_523485

AAF52269

Polypeptides with Identical Sequences

There is only one protein coding transcript and one polypeptide associated with this gene

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

Antibody raised to C-terminal peptide -ASSAKKKKKTRRIEK

(Marrus et al., 2004)

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

PDB - An information portal to biological macromolecular structures

4WXJ

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\GluRIIB using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

GluRIIB_1

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-0.19

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage

larval visceral muscle cell

(Petersen et al., 1997)

embryonic stage 12 -- 17

larval somatic muscle cell

(Petersen et al., 1997)

Additional Descriptive Data

GluRIIB transcripts are expressed exclusively in muscle. They are first detected in late stage 12 and reach maximal levels in stage 14 of embryogenesis. They are still present in the somatic musculature in stages 15-17 but at lower levels. GluRIIB transcripts are also detected at low levels in gut-associated muscle.

(Petersen et al., 1997)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

epitope tag

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 17

embryonic/larval neuromuscular junction

(Saitoe et al., 2001)

immunolocalization

Stage

Tissue/Position (including subcellular localization)

Reference

third instar larval stage

somatic muscle cell

• neuromuscular junction

(Marrus et al., 2004)

somatic muscle cell

• type I terminal bouton

(Marrus et al., 2004)

somatic muscle cell

• type Is terminal bouton

(Marrus et al., 2004)

somatic muscle cell

• type Ib terminal bouton

(Marrus et al., 2004)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

part_of ionotropic glutamate receptor complex

inferred from mutant phenotype

(Qin et al., 2005)

located_in muscle cell postsynaptic specialization

inferred from direct assay

(Qin et al., 2005)

located_in neuromuscular junction

inferred from direct assay

(Jantrapirom et al., 2019, Karr et al., 2009, Kohsaka et al., 2007, Petersen et al., 1997)

located_in postsynaptic membrane

inferred from direct assay

(Kohsaka et al., 2007)

Expression Deduced from Reporters

Reporter: Mi{Trojan-GAL4.2}GluRIIB^{MI03631-TG4.2}

Stage

Tissue/Position (including subcellular localization)

Reference

larval stage

embryonic/larval hypodermal muscle cell

(Diao et al., 2015)

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\GluRIIB in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\GluRIIB

View exonic expression by tissue for Dmel\GluRIIB

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

33789/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0020429

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0020429

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0020429

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG7234

Flygut - An atlas of the Drosophila adult midgut

FBgn0020429

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 11 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 16 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of GluRIIB

Transgenic constructs containing regulatory region of GluRIIB

Aberrations (Deficiencies and Duplications) ( 6 )

Inferred from experimentation ( 6 )

Gene disrupted in

Df(2L)cl-h4

(Sigrist et al., 2002)

(Ryder, 2004.4.26)

(Ryder, 2004.4.26)

(Ryder, 2004.4.26)

(DiAntonio et al., 1999)

Gene partially disrupted in

Df(2L)AD1

(DiAntonio et al., 1999)

Inferred from location ( 7 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

viable, with Dcr-2^UAS.cDa, Scer\GAL4^e22c, Scer\GAL4^sqh.PW

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

GluRIIB^GD917

viable, with Scer\GAL4^Mef2.PR

GluRIIB^GD917

viable, with Scer\GAL4^pnr-MD237

GluRIIB^GD917

viable, with Scer\GAL4^tin.CΔ4

GluRIIB^GD917

Other Phenotypes

Allele

abnormal neuroanatomy, with Scer\GAL4^Mhc.PW

GluRIIB^UAS.cDa

abnormal neurophysiology

GluRIIB^unspecified

abnormal neurophysiology | larval stage

abnormal neurophysiology | larval stage, with Scer\GAL4^G14

GluRIIB^UAS.cPa

wild-type

Phenotype manifest in

Allele

embryonic/larval neuromuscular junction | larval stage

GluRIIB^SP5

embryonic/larval neuromuscular junction | larval stage, with Scer\GAL4^G14

GluRIIB^UAS.cPa

motor neuron | larval stage

GluRIIB^SP5

motor neuron | larval stage, with Scer\GAL4^G14

GluRIIB^UAS.cPa

neuromuscular junction

GluRIIB^Mhc.PD

neuromuscular junction, with Scer\GAL4^how-24B

GluRIIB^UAS.cDa

neuromuscular junction, with Scer\GAL4^Mhc.PW

GluRIIB^UAS.cDa

neuromuscular junction | larval stage

GluRIIB^Mhc.Tag:MYC

neuromuscular junction | larval stage, with Scer\GAL4^Mhc.PW

GluRIIB^UAS.cDa

type Ib motor neuron | larval stage

GluRIIB^SP5

type Is motor neuron | larval stage

GluRIIB^SP5

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (21)

Hsap\GRIK4

6 of 14

Yes

5 of 14

5 of 14

5 of 14

5 of 14

5 of 14

5 of 14

4 of 14

4 of 14

Yes

4 of 14

4 of 14

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

Yes

Hsap\GRIN3B

3 of 14

Yes

2 of 14

1 of 14

1 of 14

1 of 14

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (18)

Rnor\Grik4

6 of 14

Yes

5 of 14

5 of 14

5 of 14

5 of 14

5 of 14

4 of 14

4 of 14

4 of 14

Yes

4 of 14

3 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

Mus musculus (laboratory mouse) (18)

Mmus\Grik4

6 of 14

Yes

5 of 14

5 of 14

5 of 14

5 of 14

5 of 14

4 of 14

4 of 14

4 of 14

Yes

4 of 14

4 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

Xenopus tropicalis (Western clawed frog) (31)

Xtro\grik3

3 of 13

Yes

Xtro\grik4

3 of 13

Yes

Xtro\grik5

3 of 13

Yes

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

Xtro\gmpr

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

Xtro\lrfn5

1 of 13

Xtro\lrrn4cl

1 of 13

Yes

Xtro\mettl4

1 of 13

Xtro\serpinb1

1 of 13

Xtro\vtcn1

1 of 13

Yes

Danio rerio (Zebrafish) (33)

Drer\grik4

6 of 14

Yes

5 of 14

5 of 14

5 of 14

5 of 14

5 of 14

5 of 14

5 of 14

5 of 14

5 of 14

4 of 14

4 of 14

4 of 14

4 of 14

Yes

4 of 14

4 of 14

4 of 14

3 of 14

3 of 14

Yes

Drer\grin3ba

3 of 14

Yes

Drer\LOC100331381

3 of 14

Yes

Drer\LOC110438919

3 of 14

Drer\si:ch211-251b21.1

3 of 14

Drer\si:dkey-183j2.10

3 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

Caenorhabditis elegans (Nematode, roundworm) (17)

Cele\glr-3

5 of 14

Yes

Cele\glr-5

5 of 14

Yes

Cele\glr-1

4 of 14

Cele\glr-4

4 of 14

Cele\glr-6

4 of 14

Yes

Cele\glr-2

3 of 14

Cele\glr-7

3 of 14

Cele\glr-8

3 of 14

Yes

Cele\nmr-1

2 of 14

Cele\W02A2.5

2 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Cele\T25E4.2

1 of 14

Cele\ZK867.2

1 of 14

Anopheles gambiae (African malaria mosquito) (42)

Agam\AgaP_AGAP000803

5 of 12

Yes

Agam\AgaP_AGAP012447

5 of 12

Yes

4 of 12

4 of 12

3 of 12

3 of 12

Yes

3 of 12

3 of 12

3 of 12

3 of 12

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Arabidopsis thaliana (thale-cress) (20)

Atha\ATGLR1.2

6 of 13

Yes

Atha\GLR1.1

6 of 13

Yes

Atha\GLR1.3

6 of 13

Yes

Atha\GLR1.4

6 of 13

Yes

Atha\GLR2.1

6 of 13

Yes

Atha\GLR2.2

6 of 13

Yes

Atha\GLR2.3

6 of 13

Yes

Atha\GLR2.4

6 of 13

Yes

Atha\GLR2.5

6 of 13

Yes

Atha\GLR2.6

6 of 13

Yes

Atha\GLR2.7

6 of 13

Yes

Atha\GLR2.8

6 of 13

Yes

Atha\GLR2.9

6 of 13

Yes

5 of 13

5 of 13

5 of 13

5 of 13

5 of 13

5 of 13

5 of 13

Saccharomyces cerevisiae (Brewer's yeast) (0)

Schizosaccharomyces pombe (Fission yeast) (0)

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:GluRIIB. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (43)

8 of 13

8 of 13

8 of 13

7 of 13

7 of 13

7 of 13

7 of 13

7 of 13

6 of 13

6 of 13

6 of 13

6 of 13

5 of 13

5 of 13

4 of 13

4 of 13

4 of 13

4 of 13

4 of 13

4 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 1 )

Human Ortholog

Disease

Evidence

References

GRID2; glutamate ionotropic receptor delta type subunit 2

model of autosomal recessive spinocerebellar ataxia 18

IEA

(FlyBase, 2019-)

Modifiers Based on Experimental Evidence ( 2 )

Allele

Disease

Interaction

References

GluRIIB^EGFP

ameliorates Alexander disease

modeled by Hsap\GFAP^R79H.UAS

(Wang et al., 2011)

GluRIIB^JF03145

exacerbates atypical teratoid rhabdoid tumor

modeled by Snr1^KK101602

(Tegeder et al., 2019)

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

GRIK4; glutamate ionotropic receptor kainate type subunit 4

6 of 14

600282

GRIA1; glutamate ionotropic receptor AMPA type subunit 1

5 of 14

138248

GRIA2; glutamate ionotropic receptor AMPA type subunit 2

5 of 14

138247

NEURODEVELOPMENTAL DISORDER WITH LANGUAGE IMPAIRMENT AND BEHAVIORAL ABNORMALITIES; NEDLIB

GRIA3; glutamate ionotropic receptor AMPA type subunit 3

5 of 14

305915

INTELLECTUAL DEVELOPMENTAL DISORDER, X-LINKED, SYNDROMIC, WU TYPE; MRXSW

syndromic X-linked intellectual disability 94

GRIA4; glutamate ionotropic receptor AMPA type subunit 4

5 of 14

138246

NEURODEVELOPMENTAL DISORDER WITH OR WITHOUT SEIZURES AND GAIT ABNORMALITIES; NEDSGA

GRIK1; glutamate ionotropic receptor kainate type subunit 1

5 of 14

138245

GRIK3; glutamate ionotropic receptor kainate type subunit 3

5 of 14

138243

GRID1; glutamate ionotropic receptor delta type subunit 1

4 of 14

610659

GRID2; glutamate ionotropic receptor delta type subunit 2

4 of 14

602368

SPINOCEREBELLAR ATAXIA, AUTOSOMAL RECESSIVE 18; SCAR18

autosomal recessive spinocerebellar ataxia 18

GRIK2; glutamate ionotropic receptor kainate type subunit 2

4 of 14

138244

autosomal recessive intellectual developmental disorder 6

GRIK5; glutamate ionotropic receptor kainate type subunit 5

4 of 14

600283

GRIN2A; glutamate ionotropic receptor NMDA type subunit 2A

3 of 14

138253

EPILEPSY, FOCAL, WITH SPEECH DISORDER AND WITH OR WITHOUT IMPAIRED INTELLECTUAL DEVELOPMENT; FESD

Landau-Kleffner syndrome

GRIN2B; glutamate ionotropic receptor NMDA type subunit 2B

3 of 14

138252

GRIN2C; glutamate ionotropic receptor NMDA type subunit 2C

3 of 14

138254

GRIN2D; glutamate ionotropic receptor NMDA type subunit 2D

3 of 14

602717

DEVELOPMENTAL AND EPILEPTIC ENCEPHALOPATHY 46; DEE46

developmental and epileptic encephalopathy 46

GRIN3A; glutamate ionotropic receptor NMDA type subunit 3A

3 of 14

606650

GRIN3B; glutamate ionotropic receptor NMDA type subunit 3B

3 of 14

606651

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

GluRIIB - Neto

anti tag coimmunoprecipitation, western blot

(Kim et al., 2012)

RNA-RNA

Physical Interaction

Assay

References

GluRIIB - mir-284

immunohistochemistry

(Karr et al., 2009)

Summary of Genetic Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

GluRIIB

suppressible

GluRIIA

(Sigrist et al., 2002)

GluRIIA|GluRIIB

enhanceable

VGlut1

(Han et al., 2023)

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

brm

suppressible

GluRIIB

(Tegeder et al., 2019)

Snr1

suppressible

GluRIIB

(Tegeder et al., 2019)

External Data

Linkouts

DroID - A comprehensive database of gene and protein interactions.

FBgn0020429

MIST (genetic) - An integrated Molecular Interaction Database

33789

MIST (protein-protein) - An integrated Molecular Interaction Database

33789

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyBase

External Links

External Data

Linkouts

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Class of Gene

nuclear_gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

2-17

Cytogenetic map

25E6-25E6

Sequence location

2L:5,559,342..5,563,502 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

25E6-25E6

Limits computationally determined from genome sequence between P{lacW}Hel25E^k11511 and P{lacW}l(2)k06502^k06502

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

25F-25F

(Petersen, 1998.1.20)

25F-25F

(determined by in situ hybridisation)

(Petersen et al., 1997)

Experimentally Determined Recombination Data

Location

2-17

(FlyBase, 2016)

2-18.5

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (11)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

64257

w^*; P{UAS-GluRIIB.D}3

64259

w^*; P{Mhc.GluRIIB.Myc}3

64200

w^*; GluRIIB^AD1/CyO

28718

y¹ v¹; P{TRiP.JF03145}attP2

61770

y¹ w^67c23; Mi{PT-GFSTF.2}GluRIIB^{MI03631-GFSTF.2}

37066

y¹ w^*; Mi{MIC}MI03631a Mi{MIC}MI03631b GluRIIB^MI03631

60333

y¹ w^*; Mi{Trojan-GAL4.2}GluRIIB^{MI03631-TG4.2}; Dr¹/TM3, Sb¹

NIG-Fly

2LG-2067

y² VhaAC39-1^cho-1 v¹; P{U6-GluRIIB.NIG.sgRNA}attP40/CyO

VDRC

v105581

P{KK105825}VIE-260B

v7878

w¹¹¹⁸; P{GD917}v7878

v342700

P{hsFLP}1, y1 w1118; P{HD_CFD01250}attP40/CyO-GFP

Genomic Clones (11)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (2)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

Other clones

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0020429

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0020429

Antibody Information

Laboratory Generated Antibodies

polyclonal

(Qiu et al., 2025, Kim et al., 2021, Tu et al., 2017, Ramos et al., 2015, Marrus et al., 2004)

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

GluRIIC is required for synaptic localisation of GluRIIA and GluRIIB proteins. Either GluRIIA or GluRIIB, but not both, is required for synaptic localisation of GluRIIC protein. GluRIIA and GluRIIB proteins compete with each other for access to GluRIIC protein and subsequent localisation to the synapse.

(Marrus et al., 2004)

Animals doubly mutant for GluRIIA and GluRIIB show late embryonic lethality, which can be rescued by transgenes expressing either gene alone.

(DiAntonio et al., 1997)

Glutamate receptor channels in Drosophila embryos and larvae have been studied using the patch-clamp technique.

(Nishikawa and Kidokoro, 1995)

Relationship to Other Genes

Source for database merge of

Additional comments

Nomenclature History

Source for database identify of

Source for identity of: GluRIIB CG7234

(Skupski, 1999.11)

Nomenclature comments

Etymology

Synonyms and Secondary IDs (14)

Reported As

Symbol Synonym

CG7234

(Mao et al., 2024, National Institute of Genetics Fly Stocks, 2022-, Kondo et al., 2020, Hu et al., 2015, Tsubouchi et al., 2012, Croset et al., 2010, Benton et al., 2009, Keleman et al., 2009.8.5, Perkins et al., 2009)

DGluIIB

(DiAntonio et al., 1997)

DGluR-IIB

(Saitoe et al., 2001)

DGluRIIB

(Slater, 2024, Jantrapirom et al., 2019, Lee et al., 2014, Owald et al., 2010, Lee et al., 2008, Wairkar et al., 2008, van der Plas et al., 2006, Guerrero et al., 2005, Griffith, 2004, Marrus et al., 2004, Rivlin et al., 2004, Saitoe et al., 2001, DiAntonio et al., 1999, Davis and Goodman, 1998, Davis et al., 1998, Petersen, 1998.1.20, Petersen et al., 1997)

DmelGluRIIB

(Croset et al., 2010)

Glu-RIIB

GluRIIB

(Bischoff et al., 2025, Nair et al., 2025, Neisch et al., 2025, Chen et al., 2024, Han et al., 2024, Deshpande et al., 2022, Muttathukunnel et al., 2022, Vicidomini and Serpe, 2022, Goel and Dickman, 2021, Kim et al., 2021, Nair et al., 2021, Scaplen and Petruccelli, 2021, Aponte-Santiago et al., 2020, Chou et al., 2020, Han et al., 2020, Kiragasi et al., 2020, Kondo et al., 2020, Lecompte et al., 2020, Rushton et al., 2020, Yu et al., 2020, Zhao et al., 2020, Goel et al., 2019, Hall et al., 2019, Huang et al., 2019, Metwally et al., 2019, Patrón et al., 2019, Tegeder et al., 2019, Akbergenova et al., 2018, Xing et al., 2018, Mallik et al., 2017, Perry et al., 2017, Van Vactor and Sigrist, 2017, Wang et al., 2017, Zhang et al., 2017, Zhang et al., 2017, Harris et al., 2016, Lee and Schwarz, 2016, Sturgeon et al., 2016, Wang et al., 2016, Ziegler et al., 2016, Deivasigamani et al., 2015, Diao et al., 2015, Gene Disruption Project members, 2015-, Hu et al., 2015, Kim et al., 2015, Koles et al., 2015, Muhammad et al., 2015, Ramos et al., 2015, Gardiol and St Johnston, 2014, Ghosh et al., 2014, Machamer et al., 2014, Petzoldt et al., 2014, Sulkowski et al., 2014, Xing et al., 2014, Castellanos et al., 2013, Lee et al., 2013, Shi et al., 2013, Staples and Broadie, 2013, Chen and Ganetzky, 2012, Chorna and Hasan, 2012, Fukui et al., 2012, Jordán-Álvarez et al., 2012, Kim et al., 2012, Owald et al., 2012, Owald et al., 2012, Tsai et al., 2012, Tsubouchi et al., 2012, Cheng et al., 2011, Ganesan et al., 2011, Pilgram et al., 2011, Sun et al., 2011, Wang et al., 2011, Weyhersmüller et al., 2011, Chen et al., 2010, Croset et al., 2010, Higashi-Kovtun et al., 2010, Kong et al., 2010, Morimoto et al., 2010, Nahm et al., 2010, Wang et al., 2010, Benton et al., 2009, Chen et al., 2009, Giagtzoglou et al., 2009, Karr et al., 2009, Lee et al., 2009, Menon et al., 2009, Ramachandran et al., 2009, Chai et al., 2008, Schmid et al., 2008, Augustin et al., 2007, Haas et al., 2007, Kohsaka et al., 2007, Pan and Broadie, 2007, Schmid et al., 2006, Chen and Featherstone, 2005, Chen et al., 2005, Rasse et al., 2005)

GluRIIb

(Krishnan et al., 2001)

dGluRIIB

(Ma et al., 2001)

dglurIIB

(Sigrist et al., 2002)

glurIIB

(Upadhyay et al., 2017, Featherstone et al., 2001)

Name Synonyms

Glutamate receptor IIB

(Tsubouchi et al., 2012)

glutamate receptor

(Verstreken and Bellen, 2002)

glutamate receptor IIB

(Lee et al., 2013)

Secondary FlyBase IDs

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 39 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

33789

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

Q9VMP3

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

Q9VMP3

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

33789/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0020429

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0020429

FlyMine - An integrated database for Drosophila genomics

FBgn0020429

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG7234

MARRVEL_MODEL - MARRVEL (model organism gene)

33789

PDB - An information portal to biological macromolecular structures

4WXJ

Linkouts

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0020429

DroID - A comprehensive database of gene and protein interactions.

FBgn0020429

DRSC - Results frm RNAi screens

FBgn0020429

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

GluRIIB_1

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0020429

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0020429

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG7234

Flygut - An atlas of the Drosophila adult midgut

FBgn0020429

FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.

FBgn0020429

Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution

Interactive Fly

MIST (genetic) - An integrated Molecular Interaction Database

33789

MIST (protein-protein) - An integrated Molecular Interaction Database

33789

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

References (179)

The following external sites may use different assemblies or annotations than FlyBase.

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