FlyBase Gene Report: Dmel\Ir8a

General Information

Symbol

Dmel\Ir8a

Species

D. melanogaster

Name

Ionotropic receptor 8a

Annotation Symbol

CG32704

Feature Type

protein_coding_gene

FlyBase ID

FBgn0052704

Gene Model Status

Current

Stock Availability

16 publicly available

Gene Summary

Ionotropic receptor 8a (Ir8a) encodes a member of the Ionotropic Receptor (IR) family of variant ionotropic glutamate receptors. It functions as a co-receptor together with ligand-specific IRs (in a probable heterotetrameric complex) to detect diverse organic acids. [Date last reviewed: 2019-03-07] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
Gene Group
UniProtKB
Interactive Fly

Key Links

Genomic Location

Cytogenetic map

8D2-8D2

Sequence location

X:9,232,726..9,236,585 [-]

Recombination map

1-27

RefSeq locus

NC_004354 REGION:9232726..9236585

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (25 terms)

Molecular Function (9 terms)

Terms Based on Experimental Evidence (4 terms)

CV Term

Evidence

References

enables coreceptor activity

inferred from direct assay

(Abuin et al., 2011)

contributes_to extracellular ligand-gated monoatomic ion channel activity

inferred from direct assay

(Ai et al., 2013, Abuin et al., 2011)

contributes_to extracellular phenylacetaldehyde-gated monoatomic ion channel activity

inferred from direct assay

(Abuin et al., 2011)

contributes_to olfactory receptor activity

inferred from direct assay

(Ai et al., 2013)

Terms Based on Predictions or Assertions (5 terms)

CV Term

Evidence

References

enables glutamate receptor activity

inferred from biological aspect of ancestor with PANTHER:PTN001826301

(GO Reference Genome Project, 2011-)

enables ligand-gated monoatomic ion channel activity

inferred from electronic annotation with InterPro:IPR001320, InterPro:IPR019594

(InterPro Project Members, 2004-)

inferred from sequence model

(Benton et al., 2009)

enables monoatomic ion channel activity

inferred from electronic annotation with InterPro:IPR001508

(InterPro Project Members, 2004-)

enables signaling receptor activity

inferred from electronic annotation with InterPro:IPR001508

(InterPro Project Members, 2004-)

enables transmitter-gated monoatomic ion channel activity involved in regulation of postsynaptic membrane potential

inferred from biological aspect of ancestor with PANTHER:PTN001826301

(GO Reference Genome Project, 2011-)

Biological Process (10 terms)

Terms Based on Experimental Evidence (7 terms)

CV Term

Evidence

References

involved_in cellular response to acetate

inferred from direct assay

(Ai et al., 2013)

involved_in detection of chemical stimulus involved in sensory perception of smell

inferred from mutant phenotype

(Abuin et al., 2011)

involved_in detection of chemical stimulus involved in sensory perception

inferred from expression pattern

(Benton et al., 2009)

involved_in olfactory behavior

inferred from mutant phenotype

(Ai et al., 2013)

involved_in positive regulation of calcium-mediated signaling

inferred from mutant phenotype

(Ai et al., 2013)

involved_in response to acetate

inferred from mutant phenotype

(Ai et al., 2013)

involved_in response to chlorate

inferred from mutant phenotype

(Ai et al., 2013)

Terms Based on Predictions or Assertions (3 terms)

CV Term

Evidence

References

involved_in modulation of chemical synaptic transmission

inferred from biological aspect of ancestor with PANTHER:PTN001826301

(GO Reference Genome Project, 2011-)

involved_in monoatomic ion transport

inferred from electronic annotation with InterPro:IPR001508

(InterPro Project Members, 2004-)

involved_in synaptic transmission, glutamatergic

inferred from biological aspect of ancestor with PANTHER:PTN001826301

(GO Reference Genome Project, 2011-)

Cellular Component (6 terms)

Terms Based on Experimental Evidence (4 terms)

CV Term

Evidence

References

located_in dendrite membrane

inferred from direct assay

(Ai et al., 2013)

located_in plasma membrane

inferred from direct assay

(Abuin et al., 2011)

part_of receptor complex

inferred from direct assay

(Abuin et al., 2011)

located_in sensory dendrite

inferred from direct assay

(Ai et al., 2013, Abuin et al., 2011)

Terms Based on Predictions or Assertions (3 terms)

CV Term

Evidence

References

located_in membrane

inferred from sequence model

(Benton et al., 2009)

inferred from electronic annotation with InterPro:IPR001320, InterPro:IPR001508, InterPro:IPR019594

(InterPro Project Members, 2004-)

is_active_in plasma membrane

inferred from biological aspect of ancestor with PANTHER:PTN000437926

(GO Reference Genome Project, 2011-)

is_active_in postsynaptic density membrane

inferred from biological aspect of ancestor with PANTHER:PTN001826301

(GO Reference Genome Project, 2011-)

Gene Group (FlyBase)

ANTENNAL IONOTROPIC CO-RECEPTORS

Protein Family (UniProt)

Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. (Q9W365)

Protein Signatures (InterPro)

Summaries

Gene Snapshot

Gene Group (FlyBase)

ANTENNAL IONOTROPIC CO-RECEPTORS -: Ionotropic Receptors (IR) co-receptors are part of the IR family. They are broadly, but selectively, expressed in antennal sensory neurons in Drosophila. Some antenneal IRs only form functional odor-responsive ion channels as a heteromeric complex with an IR co-receptor. (Adapted from FBrf0222310).

Protein Function (UniProtKB)

Ionotropic receptor coreceptor, which is part of a neural sensory system involved in male courtship (PubMed:19135896, PubMed:21220098, PubMed:38114448). Coreceptors complex with conventional ionotropic receptors to form chemosensory units, providing sensitive and prolonged signaling (PubMed:21220098). Together with Ir84a, Ir8a is an integral part of the functional ionotropic receptors involved in male courtship in response to food-derived odors, such as phenylacetic acid and phenylacetaldehyde, which are widely found in fruit and other plant tissues that serve as food sources and oviposition sites (PubMed:38114448).

(UniProt, Q9W365)

Summary (Interactive Fly)

a member of a novel family of chemosensory receptor ion channels - variant ionotropic glutamate receptor-like protein - expressed on antennal neurons

Interactive Fly

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\Ir8a for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry Q9W365)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Gene model reviewed during 5.52

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

Ir8a-RA

FBtr0071333

NM_167185

2811

936

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

Ir8a-PA

FBpp0071268

106.3

936

5.53

Q9W365

NP_727328

AAF46470

Polypeptides with Identical Sequences

There is only one protein coding transcript and one polypeptide associated with this gene

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Subunit Structure (UniProtKB)

Heterodimer with conventional ionotropic receptors, such as Ir84a, to form functional ionotropic receptor units.

(UniProt, Q9W365)

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Q9W365

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Ir8a using the Feature Mapper tool.

External Data

Crossreferences

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

NA

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult antennal sense organ | restricted

(Benton et al., 2009)

neuron of coeloconic sensillum of antennal segment 3

(Benton et al., 2009)

RT-PCR

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

antenna

(Croset et al., 2010)

Additional Descriptive Data

Ir8a transcript is expressed in the neurons of the sensillum coeloconicum of antennal segment 3 located throughout the antenna and around the sacculus.

(Benton et al., 2009)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

immunolocalization

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

neuron of coeloconic sensillum of antennal segment 3 | subset

• cell body

(Abuin et al., 2011)

neuron of coeloconic sensillum of antennal segment 3 | subset

• dendrite terminus

(Abuin et al., 2011)

neuron of sensillum of sacculus chamber III

(Abuin et al., 2011)

adult olfactory receptor neuron Ir64a

(Ai et al., 2013)

western blot

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

antenna

(Ai et al., 2013)

Additional Descriptive Data

Ir8a protein is expressed in the cell body and dendritic ends of a subset of neurons of sensillum coeloconicum of antennal segment 3 and in the neurons of the grooved sensillum of sacculus, which project to the third chamber of the sacculus. A subset of the sensilla coeloconicum labeled with Ir8a also expresses Ir25a.

(Abuin et al., 2011)

Marker for

Subcellular Localization

CV Term

Evidence

References

located_in dendrite membrane

inferred from direct assay

(Ai et al., 2013)

located_in plasma membrane

inferred from direct assay

(Abuin et al., 2011)

part_of receptor complex

inferred from direct assay

(Abuin et al., 2011)

located_in sensory dendrite

inferred from direct assay

(Ai et al., 2013, Abuin et al., 2011)

Expression Deduced from Reporters

Reporter: P{Ir8a-GAL4.A}

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

neuron of coeloconic sensillum of antennal segment 3 | subset

• cell body

(Abuin et al., 2011)

neuron of coeloconic sensillum of antennal segment 3 | subset

• cilium

(Abuin et al., 2011)

neuron of coeloconic sensillum of antennal segment 3 | subset

• axon

(Abuin et al., 2011)

neuron of antennal coeloconic sensillum ac1

(Silbering et al., 2011)

neuron of antennal coeloconic sensillum ac2

(Silbering et al., 2011)

neuron of antennal coeloconic sensillum ac3

(Silbering et al., 2011)

neuron of antennal coeloconic sensillum ac4

(Silbering et al., 2011)

neuron of sensillum of sacculus chamber III

(Silbering et al., 2011)

adult antennal lobe glomerulus | subset

(Silbering et al., 2011)

chamber II of sacculus

(Sun et al., 2018)

Reporter: P{Ir8a-GAL4.Ab}

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult olfactory receptor neuron Ir64a

(Ai et al., 2013)

antennal lobe glomerulus DP1m

(Ai et al., 2013)

antennal lobe glomerulus DC4

(Ai et al., 2013)

adult antennal lobe glomerulus | restricted

(Ai et al., 2013)

Reporter: P{Ir8a-GAL4.M}

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult olfactory receptor neuron Ir31a

(Menuz et al., 2014)

adult olfactory receptor neuron Ir75a

(Menuz et al., 2014)

adult olfactory receptor neuron Ir75b

(Menuz et al., 2014)

adult olfactory receptor neuron Ir84a

(Menuz et al., 2014)

adult olfactory receptor neuron Ir64a

(Menuz et al., 2014)

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\Ir8a in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\Ir8a

View exonic expression by tissue for Dmel\Ir8a

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

31867/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0052704

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0052704

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0052704

Flygut - An atlas of the Drosophila adult midgut

FBgn0052704

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 4 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 19 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Ir8a

Transgenic constructs containing regulatory region of Ir8a

Aberrations (Deficiencies and Duplications) ( 0 )

Inferred from experimentation ( 0 )

Inferred from location ( 17 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

viable

Ir8a¹

viable, with Dcr-2^UAS.cDa, Scer\GAL4^e22c, Scer\GAL4^sqh.PW

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

viable, with Scer\GAL4^Mef2.PR

Ir8a^GD15330

viable, with Scer\GAL4^pnr-MD237

Ir8a^GD15330

Sterility

Allele

fertile

Ir8a¹

Other Phenotypes

Allele

abnormal neuroanatomy | adult stage | progressive

Ir8a¹

abnormal neurophysiology

Ir8a¹

abnormal smell perception

Ir8a¹

abnormal smell perception, with Scer\GAL4^Ir64a.PA

decreased cell number | adult stage | progressive

Ir8a¹

Phenotype manifest in

Allele

adult olfactory receptor neuron Ir64a | decreased number

Ir8a¹

coeloconic sensillum of antennal segment 3

Ir8a¹

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (19)

Hsap\GRIA1

4 of 14

Yes

Hsap\GRIA2

4 of 14

Yes

Hsap\GRIA3

4 of 14

Yes

Hsap\GRIA4

4 of 14

Yes

Hsap\GRIK4

4 of 14

Yes

3 of 14

3 of 14

3 of 14

3 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (17)

Rnor\Gria3

4 of 14

Yes

Rnor\Gria4

4 of 14

Yes

Rnor\Grik4

4 of 14

Yes

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

Mus musculus (laboratory mouse) (17)

Mmus\Gria1

4 of 14

Yes

Mmus\Gria2

4 of 14

Yes

Mmus\Gria3

4 of 14

Yes

Mmus\Gria4

4 of 14

Yes

Mmus\Grik4

4 of 14

Yes

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

Xenopus tropicalis (Western clawed frog) (21)

Xtro\grik4

3 of 13

Yes

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

Xtro\grm5

1 of 13

Xtro\serpinb1

1 of 13

Danio rerio (Zebrafish) (33)

Drer\gria1a

4 of 14

Yes

Drer\gria1b

4 of 14

Yes

Drer\gria2a

4 of 14

Yes

Drer\gria2b

4 of 14

Yes

Drer\gria3a

4 of 14

Yes

Drer\gria3b

4 of 14

Yes

Drer\gria4b

4 of 14

Yes

Drer\grik4

4 of 14

Yes

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

Drer\si:ch211-251b21.1

3 of 14

Drer\si:dkey-183j2.10

3 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Caenorhabditis elegans (Nematode, roundworm) (16)

Cele\glr-1

4 of 14

Yes

Cele\glr-3

4 of 14

Yes

Cele\glr-5

4 of 14

Yes

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

Yes

Cele\nmr-1

2 of 14

Cele\W02A2.5

2 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Cele\T25E4.2

1 of 14

Cele\ZK867.2

1 of 14

Anopheles gambiae (African malaria mosquito) (45)

Agam\AgaP_AGAP010411

10 of 12

Yes

4 of 12

3 of 12

3 of 12

3 of 12

Yes

3 of 12

3 of 12

3 of 12

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Arabidopsis thaliana (thale-cress) (20)

Atha\ATGLR1.2

6 of 13

Yes

Atha\GLR1.1

6 of 13

Yes

Atha\GLR1.3

6 of 13

Yes

Atha\GLR1.4

6 of 13

Yes

Atha\GLR2.1

6 of 13

Yes

Atha\GLR2.2

6 of 13

Yes

Atha\GLR2.3

6 of 13

Yes

Atha\GLR2.4

6 of 13

Yes

Atha\GLR2.5

6 of 13

Yes

Atha\GLR2.6

6 of 13

Yes

Atha\GLR2.7

6 of 13

Yes

Atha\GLR2.8

6 of 13

Yes

Atha\GLR2.9

6 of 13

Yes

Atha\GLR2

6 of 13

Yes

Atha\GLR3.3

6 of 13

Yes

Atha\GLR3.4

6 of 13

Yes

Atha\GLR3.5

6 of 13

Yes

Atha\GLR3.6

6 of 13

Yes

Atha\GLR5

6 of 13

Yes

Atha\GLUR2

6 of 13

Yes

Saccharomyces cerevisiae (Brewer's yeast) (0)

Schizosaccharomyces pombe (Fission yeast) (0)

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:Ir8a. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (45)

7 of 13

6 of 13

6 of 13

6 of 13

6 of 13

6 of 13

6 of 13

6 of 13

5 of 13

5 of 13

5 of 13

5 of 13

5 of 13

4 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

mtt

1 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 3 )

Human Ortholog

Disease

Evidence

References

GRIA1; glutamate ionotropic receptor AMPA type subunit 1

model of autosomal dominant intellectual developmental disorder 67

IEA

(FlyBase, 2019-)

model of autosomal recessive intellectual developmental disorder 76

IEA

(FlyBase, 2019-)

GRIA3; glutamate ionotropic receptor AMPA type subunit 3

model of syndromic X-linked intellectual disability 94

IEA

(FlyBase, 2019-)

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

GRIA1; glutamate ionotropic receptor AMPA type subunit 1

4 of 14

138248

GRIA2; glutamate ionotropic receptor AMPA type subunit 2

4 of 14

138247

NEURODEVELOPMENTAL DISORDER WITH LANGUAGE IMPAIRMENT AND BEHAVIORAL ABNORMALITIES; NEDLIB

GRIA3; glutamate ionotropic receptor AMPA type subunit 3

4 of 14

305915

INTELLECTUAL DEVELOPMENTAL DISORDER, X-LINKED, SYNDROMIC, WU TYPE; MRXSW

syndromic X-linked intellectual disability 94

GRIA4; glutamate ionotropic receptor AMPA type subunit 4

4 of 14

138246

NEURODEVELOPMENTAL DISORDER WITH OR WITHOUT SEIZURES AND GAIT ABNORMALITIES; NEDSGA

GRIK4; glutamate ionotropic receptor kainate type subunit 4

4 of 14

600282

GRID1; glutamate ionotropic receptor delta type subunit 1

3 of 14

610659

GRID2; glutamate ionotropic receptor delta type subunit 2

3 of 14

602368

SPINOCEREBELLAR ATAXIA, AUTOSOMAL RECESSIVE 18; SCAR18

autosomal recessive spinocerebellar ataxia 18

GRIK1; glutamate ionotropic receptor kainate type subunit 1

3 of 14

138245

GRIK3; glutamate ionotropic receptor kainate type subunit 3

3 of 14

138243

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

Ir8a - Ir64a

anti tag coimmunoprecipitation, peptide massfingerprinting, western blot

(Ai et al., 2013)

Ir8a - Ir84a

fluorescence microscopy, tag visualisation

(Abuin et al., 2011)

Summary of Genetic Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Orco

suppressible

Ir8a

(Silbering et al., 2011)

External Data

Subunit Structure (UniProtKB)

Heterodimer with conventional ionotropic receptors, such as Ir84a, to form functional ionotropic receptor units.

(UniProt, Q9W365 )

Linkouts

DroID - A comprehensive database of gene and protein interactions.

FBgn0052704

MIST (protein-protein) - An integrated Molecular Interaction Database

31867

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyBase

External Links

External Data

Linkouts

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Class of Gene

nuclear_gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

1-27

Cytogenetic map

8D2-8D2

Sequence location

X:9,232,726..9,236,585 [-]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

8D2-8D2

Limits computationally determined from genome sequence between P{EP}EP1450&P{EP}EP1356 and P{EP}EP912^EP912

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

1-27

(FlyBase, 2016)

1-28.4

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (16)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

92398

w^* TI{2A-QF2}Ir8a^2A-QF2

25813

y¹ v¹; P{TRiP.JF01831}attP2

41731

w^*; P{Ir8a-GAL4.A}204.8; TM2/TM6B, Tb¹

41745

w^*; M{UAS-Ir8a.A}ZH-51D; TM2/TM6B, Tb⁺

41744

w^* TI{TI}Ir8a¹; Bl¹ L²/CyO

93790

y¹ w^* TI{CRIMIC.TG4.0}Ir8a^{CR02617-TG4.0}/FM7h

92399

w^* TI{2A-QF2}Ir8a^2A-QF2; PBac{10XQUAS-6XGFP}VK00018/CyO

98386

w^*; M{UAS-Ir8a.A}ZH-51D, TI{GAL4}Or22ab-Df; TM2/TM6B, Tb¹

98412

w^*; M{UAS-Ir8a.A}ZH-51D, TI{GAL4}Or22ab-Df; P{UAS-CpomIr64a.Δ}attP2

VDRC

v51067

w¹¹¹⁸; P{GD3293}v51067

v29917

w¹¹¹⁸; P{GD15330}v29917

v341801

P{hsFLP}1, y¹ w¹¹¹⁸; P{HD_CFD00940}attP40/CyO-GFP

More stocks available...

Genomic Clones (12)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (1)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

Other clones

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0052704

Antibody Information

Laboratory Generated Antibodies

polyclonal

(Abuin et al., 2011)

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

v is required for Ir64a protein trafficking and stability.

(Ai et al., 2013)

Relationship to Other Genes

Source for database merge of

Source for merge of: CG12122 CG12666

(FlyBase Genome Annotators, 2002-2003)

Additional comments

Annotations CG12122 and CG12666 merged as CG32704 in release 3 of the genome annotation.

(FlyBase Genome Annotators, 2002-2003)

Nomenclature History

Source for database identify of

Source for identity of: Ir8a CG32704

(Benton et al., 2009)

Nomenclature comments

Etymology

Synonyms and Secondary IDs (10)

Reported As

Symbol Synonym

(Ni, 2020)

CG12122

CG12666

CG32704

(Tsubouchi et al., 2012, Chung et al., 2011, Croset et al., 2010, Itoh et al., 2010, Benton et al., 2009, Lee et al., 2009, Perkins et al., 2009.1.15)

CT7438

(Littleton and Ganetzky, 2000)

DmelIR8a

(Croset et al., 2010)

Name Synonyms

CG32704

Ionotropic receptor 8a

(Tsubouchi et al., 2012)

ionotropic receptor 8a

(Benton et al., 2009)

Secondary FlyBase IDs

FBgn0030110
FBgn0030111

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 32 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

31867

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

AAF46470

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

Q9W365

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

Q9W365

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

Q9W365

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

31867/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0052704

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0052704

FlyMine - An integrated database for Drosophila genomics

FBgn0052704

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG32704

MARRVEL_MODEL - MARRVEL (model organism gene)

31867

Linkouts

DroID - A comprehensive database of gene and protein interactions.

FBgn0052704

DRSC - Results frm RNAi screens

FBgn0052704

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0052704

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0052704

Flygut - An atlas of the Drosophila adult midgut

FBgn0052704

FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.

FBgn0052704

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC003572

Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution

Interactive Fly

MIST (protein-protein) - An integrated Molecular Interaction Database

31867

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

References (104)

The following external sites may use different assemblies or annotations than FlyBase.

NA

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