FB2026_03 , released September 17, 2026
Recombinant Construct: P{20XUAS-lin-28.M1+M2.FLAG-HA}
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General Information
Symbol
P{20XUAS-lin-28.M1+M2.FLAG-HA}
FlyBase ID
FBtp0111008
Feature type
transgenic_transposable_element
Component Allele(s)
lin-28M1+M2.20xUAS.Tag:FLAG,Tag:HA
Size
Expression Data
Associated insertion(s)
1 available
Molecular map
Description and Uses
Construct components
Component allele lin-28M1+M2.20xUAS.Tag:FLAG,Tag:HA
Transgenic product class
missense_variant
(Chen et al., 2015)
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
lin-28
Tagged with
Tag:FLAG
Tag:HA
Description

20xUAS regulatory sequences drive expression of a mutated form of lin-28 (carries mutations in the CSD domain which convert the amino acids at positions 45, 54 and 65 to Ala residues and carries mutations in the CCHC domain that converts amino acids at positions 136 and 158 to Ala residues) which is tagged at the C-terminal end with three copies of Tag:FLAG and then three copies of Tag:HA.

(Chen et al., 2015)
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (0)
Stocks containing insertion(s) of this recombinant construct
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
P{20XUAS-lin-28.M1+M2.FLAG-HA}
(FlyBase, 1992-)
P{yw,20XUAS-Lin-28M1+M2::FLAG-HA}
(Chen et al., 2015)
UAS-Lin-28M1+M2::FLAG-HA
(Chen et al., 2015)
Secondary FlyBase IDs
    References (3)