FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Recombinant Construct: M{UAS-Ub.Asn.GFP}
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General Information
Symbol
M{UAS-Ub.Asn.GFP}
FlyBase ID
FBtp0130233
Feature type
transgenic_transposable_element
Component Allele(s)
Avic\GFPE.UAS.Ub.Asn
Size
Expression Data
Associated insertion(s)
2 available
Molecular map
Description and Uses
Construct components
Component allele Avic\GFPE.UAS.Ub.Asn
Transgenic product class
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
EGFP
Description

UASt regulates expression of a Ubiquitin-eGFP chimera with a asparagine and a linker sequence GKLGRQ between the ubiquitin- and eGFP-coding sequences. Upon proteolytic cleavage (deubiquitination), a destabilizing amino acid (asparagine) is exposed at the N-terminus of the GFP moiety, thereby acting as an unstable GFP reporter for activity of the N-end rule pathway.

(Zülbahar et al., 2018)
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (1)
Stocks containing insertion(s) of this recombinant construct
Bloomington
602909
w*; M{UAS-Ub.Asn.GFP}ZH-86Fb
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
M{UAS-Ub.Asn.GFP}
(FlyBase, 1992-)
UASt-Asn.GFP
(François et al., 2023)
Ub-Asn-GFP
(Zülbahar et al., 2018)
Secondary FlyBase IDs
    References (4)