FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Recombinant Construct: TI{αTub84B-SPARC3-I-OUT-GAL80}
Open Close
General Information
Symbol
TI{αTub84B-SPARC3-I-OUT-GAL80}
FlyBase ID
FBtp0150131
Feature type
engineered_region
Component Allele(s)
Size
Expression Data
Associated insertion(s)
Molecular map
Description and Uses
Construct components
Transgenic product class
Component
Use(s)
Regulatory region(s)
Description

A αTub84B promoter is fused upstream of a "SPARC3-I-OUT-GAL80" module that contains: an attP site of 38bp, the GAL80 repressor, a second attP site (60bp, full-length site), spacer sequence and an attB site. Ribozyme sequences are also present (to prevent leaky expression in the absence of phiC31:int). phiC31:int catalyzes irreversible recombination between the attB site and one of the two attP sites; if the attP site upstream of the GAL80 repressor is utilized, GAL80 is excised (allowing GAL4-driven expression of any effectors present in the animal), while if the attP site downstream of the GAL80 repressor is utilized, GAL80 is retained (preventing GAL4-driven expression). The recombination reaction that results in loss of GAL80 is expected to occur sporadically due to the upstream attP site in the "SPARC3-I-OUT-GAL80" module being truncated.

Sequence Data
Sequence (FB)
Associated Sequence Data
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (0)
Stocks containing insertion(s) of this recombinant construct
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (1)
Reported As
Symbol Synonym
TI{αTub84B-SPARC3-I-OUT-GAL80}
Secondary FlyBase IDs
    References (3)