P{HD_CFD01402} carries the same sgRNAs as P{HD_CFD01690}, but with the sgRNAs in reverse order.

UAS regulatory sequences drive expression of two different sgRNAs, both of which target common coding exons within the 5' half of the CG9962 gene without overlapping the start codon; this is designed to result in an indel (small insertion or deletion mutation) of the target gene in the presence of a source of Cas9. Designed for conditional CRISPR mutagenesis; a GAL4 driver can be used to express the sgRNAs and a UAS-Cas9 transgene in a tissue-specific manner, inducing mutagenesis only in the GAL4-expressing cells. P{HD_CFD01402} carries the same sgRNAs as P{HD_CFD01690}, but with the sgRNAs in reverse order.