Allele Dmel\ea²

General Information
Symbol	Dmel\ea²	Species	D. melanogaster
Name		FlyBase ID	FBal0003456
Feature type	allele	Associated gene	Dmel\ea

Allele class	amorphic allele - genetic evidence, gain of function allele
Mutagen	ethyl methanesulfonate
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class	amorphic allele - genetic evidence (Ray et al., 1991) gain of function allele (Ray et al., 1991)
Mutagen	ethyl methanesulfonate (Tearle and Nusslein-Volhard, 1987)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Point mutation in the catalytic site. (Lemaitre et al., 1996)
Cytology
Phenotypic Data
Phenotypic Class

Phenotype Manifest In
	embryonic epidermis (Carroll et al., 1987, Ray et al., 1991)
Detailed Description
	Statement Reference Bacterially challenged ea²/ea⁷ mutants exhibit a wild type Drs response. Pattern of response of CecA1 and CecA2 parallels that of Drs. Dpt and Dro remain fully inducible and pattern of expression of AttA and Def in intermediate. Infection of ea¹/ea² mutants with A.fumigatus causes higher survival rate than wild type, infection with E.coli causes 87% survival 3 days postinfection. (Lemaitre et al., 1996) Perivitelline fluid from Tl and dl donors was capable of restoring polarized gastrulation movements and cuticular elements. Perivitelline fluid transplantation using ea^D8 embryos as donors and ea¹/ea² embryos as recipients demonstrated that the rescue of the ea phenotype results from the transplantation of the ea gene product. (Stein and Nusslein-Volhard, 1992) Ventralized embryos: rings or patches of ventral denticles along dorsoventral axis. Altered pattern of dpp and zen. (Ray et al., 1991) Embryos derived from homozygous females are dorsalised. (Carroll et al., 1987)
External Data
Linkouts
Interactions
Phenotypic Class
NOT Suppressor of
Statement Reference ea² is a non-suppressor of partially lethal - majority die \| recessive phenotype of cact⁷ (Qiu et al., 1998)
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement Reference Embryos derived from Spn27A^ex32/Df(2L)BSC7 ; ea¹/ea² females are completely dorsalised. (Ligoxygakis et al., 2003) The zygotic semi-lethality of cact⁷ homozygotes is not suppressed by ea². (Qiu et al., 1998)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 1 )
Reported As
Symbol Synonym	ea² (Leclerc et al., 2006, Cho et al., 2010)
Name Synonym
Secondary FlyBase IDs

References ( 12 )
Research paper	Cho et al., 2010, Curr. Biol. 20(12): 1133--1137 Pipe-Dependent Ventral Processing of Easter by Snake Is the Defining Step in Drosophila Embryo DV Axis Formation. [FBrf0211253] Leclerc et al., 2006, EMBO Rep. 7(2): 231--235 Prophenoloxidase activation is not required for survival to microbial infections in Drosophila. [FBrf0190374] Ligoxygakis et al., 2003, Curr. Biol. 13(23): 2097--2102 A serpin regulates dorsal-ventral axis formation in the Drosophila embryo. [FBrf0167413] Qiu et al., 1998, Development 125(10): 1909--1920 A role for the Drosophila Toll/Cactus pathway in larval hematopoiesis. [FBrf0103015] Lemaitre et al., 1996, Cell 86(6): 973--983 The dorsoventral regulatory gene cassette spatzle/Toll/cactus controls the potent antifungal response in Drosophila adults. [FBrf0090665] Lemaitre et al., 1995, EMBO J. 14(3): 536--545 Functional analysis and regulation of nuclear import of dorsal during the immune response in Drosophila. [FBrf0080205] Stein and Nusslein-Volhard, 1992, Cell 68(3): 429--440 Multiple extracellular activities in Drosophila egg perivitelline fluid are required for establishment of embryonic dorsal-ventral polarity. [FBrf0055562] Ray et al., 1991, Development 113(1): 35--54 The control of cell fate along the dorsal-ventral axis of the Drosophila embryo. [FBrf0053782] Roth et al., 1991, Development 112: 371--388 cactus, a maternal gene required for proper formation of the dorsoventral morphogen gradient in Drosophila embryos. [FBrf0053786] Stein et al., 1991, Cell 65: 725--735 The polarity of the dorsoventral axis in the Drosophila embryo is defined by an extracellular signal. [FBrf0053394] Carroll et al., 1987, Development 99: 327--332 Genes that control dorsoventral polarity affect gene expression along the anteroposterior axis of the Drosophila embryo. [FBrf0046065]
Stock list	Tearle and Nusslein-Volhard, 1987, D. I. S. 66: 209--269 Tubingen mutants and stock list. [FBrf0045941]

Allele Dmel\ea2

Allele Dmel\ea²