FlyBase Allele Report: Dmel\lin[2]

General Information

Symbol

Dmel\lin²

Species

D. melanogaster

Name

FlyBase ID

FBal0011651

Feature type

allele

Associated gene

Dmel\lin

Associated Insertion(s)

Carried in Construct

Also Known As

lin^IIF, lin^IIF103, lin^2f, lines^2f

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

(Tearle and Nusslein-Volhard, 1987)

Progenitor genotype

Cytology

Description

Amino acid replacement: Q109term.

(Wen et al., 2024)

Amino acid replacement: ?109term.

(Hatini et al., 2000)

Nucleotide substitution: C?T.

(Hatini et al., 2000)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

2R:8,915,425..8,915,425 [+]

Nucleotide change:

C8915425T

Reported nucleotide change:

C?T

Amino acid change:

Q109term | lin-PA

Reported amino acid change:

?109term

(Hatini et al., 2000)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal size | embryonic stage

(Iwaki et al., 2001)

increased cell number | embryonic stage

(Iwaki et al., 2001)

Phenotype Manifest In

abdominal segment 8

(Strecker et al., 1992)

denticle | ectopic (with lin^G2)

(Hatini et al., 2000)

denticle row 1 (with lin^G2)

(Hatini et al., 2000)

denticle row 2 (with lin^G2)

(Hatini et al., 2000)

denticle row 2 | ectopic (with lin^G2), with Scer\GAL4^ptc-559.1, lin^UAS.cHa

(Hatini et al., 2000)

denticle row 3 (with lin^G2)

(Hatini et al., 2000)

denticle row 3 | ectopic (with lin^G2), with Scer\GAL4^ptc-559.1, lin^UAS.cHa

(Hatini et al., 2000)

denticle row 4 (with lin^G2)

(Hatini et al., 2000)

dorsal denticle | ectopic (with lin^G2)

(Hatini et al., 2000)

dorsal fine hair

(Bokor and DiNardo, 1996)

dorsal thick hair

(Bokor and DiNardo, 1996)

embryonic/larval hindgut | embryonic stage

(Harbecke and Lengyel, 1995)

embryonic/larval posterior spiracle

(Harbecke and Lengyel, 1995)

embryonic hindgut

(Iwaki et al., 2001)

embryonic Malpighian tubule

(Harbecke and Lengyel, 1995)

larval somatic muscle cell | dorsal

(Volk and Vijay Raghavan, 1994)

ventral denticle belt (with lin^G2)

(Hatini et al., 2000)

Detailed Description

Statement

Reference

Mutant embryos at stage 16 have hindguts about half the size of wild-type. These hindguts have larger lumens and cells in their circumference than those of wild-type. The hindgut appears distended or bloated. The "boundary cells" that normally run the length of the large intestine are missing. Cell proliferation and apoptosis appear normal in mutant hindguts. Hindguts contain slightly more cells than wild-type. The visceral mesoderm is also normal in these mutants.

(Iwaki et al., 2001)

In lin^G2/lin² embryos, the first three rows of the ventral denticle belts are replaced with smooth cuticle and denticle row 4 appears large like row 5. Excess denticles are produced posterior to the denticle belt. The anterior- and posterior-most en-expressing cells in the parasegment differentiate the 1^o dorsal cuticle type (dorsal denticles) in lin^G2/lin² embryos, while 2 to 3 cell rows internal to the en expression domain differentiate smooth cuticle. Expression of lin^Scer\UAS.cHa under the control of Scer\GAL4^en-e16E in these embryos transforms all en-expressing cells to the 4^o fate (fine hairs). Expression of lin^Scer\UAS.cHa under the control of Scer\GAL4^wg.PM restores the 4^o fate, but only in the portion of the 4^o field that expresses wg.

(Hatini et al., 2000)

In mutants 1^o and 2^o cell types are established normally in the dorsal epidermis, but 4^o cell types are completely missing. There is an expansion in the number of rows of cells that adopt a 3^o cell fate. Loss of hh function 6hrs AEL leads to loss of 3^o fates. In double mutants with lin²/lin³ the 3^o cell type is restored.

(Bokor and DiNardo, 1996)

The posterior part of the embryo is poorly differentiated; posterior spiracles are present but do not evert and occasional pale denticles of the A8 denticle belt are seen. The hindgut is shorter than normal and bloated and the stomatogastric invaginations are fused. Malpighian tubules are not oriented correctly and their lumina appear slightly bloated.

(Harbecke and Lengyel, 1995)

Pattern of embryonic dorsal muscles is disorganized. Myotubes attach to the ectopic shot-expressing cells, and are consequently shorter than wild type. The organization of muscle precursors as revealed by nau RNA expression is wild type.

(Volk and Vijay Raghavan, 1994)

Homozygous embryos lack derivatives of A8.

(Strecker et al., 1992)

Suppression of tor^13D embryos: increase in proportion of embryos that formed cuticle with denticles and decrease in proportion of embryos that formed empty sacs.

(Strecker et al., 1991)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Expression of arm^{S10.Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^ptc-559.1 in lin²/lin² embryos does not result in the global specification of 4^o cell types in the dorsal cuticle, the dorsal cuticle phenotype resembles that of lin mutants.

(Hatini et al., 2000)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Partially rescued by

lin^G2/lin² is partially rescued by Scer\GAL4^ptc-559.1/lin^UAS.cHa

(Hatini et al., 2000)

lin^G2/lin² is partially rescued by lin^UAS.cHa/Scer\GAL4^wg.PM

(Hatini et al., 2000)

Comments

Expression of lin^Scer\UAS.cHa under the control of Scer\GAL4^ptc-559.1 rescues ventral and dorsal wg-dependent cell fates in lin²/lin^G2 embryos. Anterior ventral denticle rows are restored and excess row 2/3 denticles are produced. The excess denticles posterior to the belt that are seen in lin²/lin^G2 embryos are not restored to smooth cuticle.