Allele Scer\GAL4^2-286

General Information
Symbol	Scer\GAL4^2-286	Species	S. cerevisiae
Name		FlyBase ID	FBal0065207
Feature type	allele	Associated gene	Scer\GAL4

Allele class
Mutagen	P-element activity
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	P-element activity (Timmons et al., 1997)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference
Caused by insertion	P{GawB}2-286 (Timmons et al., 1997, Huang et al., 2005, Andrews et al., 2002, Yoshiyama et al., 2006, Fluegel et al., 2006, Kaplan et al., 2008, Huang et al., 2007, Niwa et al., 2010, Phillips et al., 2008, Wang et al., 2011, Yoshiyama-Yanagawa et al., 2011)
Cytology
Phenotypic Data
Phenotypic Class
	auxotroph, with nvd^{dsRNA.IR-1.Scer\UAS} (Lang et al., 2012) developmental rate defective \| first instar larval stage, with nvd^{dsRNA.IR-1.Scer\UAS} (Yoshiyama et al., 2006) developmental rate defective \| second instar larval stage, with sro^{dsRNA.Scer\UAS} (Niwa et al., 2010) lethal \| first instar larval stage, with nvd^{dsRNA.IR-1.Scer\UAS} (Yoshiyama et al., 2006, Yoshiyama-Yanagawa et al., 2011) lethal \| first instar larval stage, with nvd^{dsRNA.IR-2.Scer\UAS} (Yoshiyama et al., 2006) lethal \| second instar larval stage, with sec10^{dsRNA.Scer\UAS} (Andrews et al., 2002) lethal \| second instar larval stage, with sro^{dsRNA.Scer\UAS} (Niwa et al., 2010) male sterile, with Npc1a^{Scer\UAS.T:Avic\GFP-EYFP}, Npc1a^unspecified (Wang et al., 2011)
Phenotype Manifest In
	individualization stage spermatid, with Npc1a^{Scer\UAS.T:Avic\GFP-EYFP}, Npc1a^unspecified (Wang et al., 2011) seminal vesicle, with Npc1a^{Scer\UAS.T:Avic\GFP-EYFP}, Npc1a^unspecified (Wang et al., 2011) sperm individualization complex, with Npc1a^{Scer\UAS.T:Avic\GFP-EYFP}, Npc1a^unspecified (Wang et al., 2011)
Detailed Description
	Statement Reference
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
Statement Reference Scer\GAL4^2-286, nvd^{dsRNA.IR-1.Scer\UAS} has auxotroph phenotype, suppressible by Dpac\nvd^{4mut.Scer\UAS}, Scer\GAL4^2-286 (Lang et al., 2012, Lang et al., 2012) Scer\GAL4^2-286, nvd^{dsRNA.IR-1.Scer\UAS} has lethal \| first instar larval stage phenotype, suppressible by Bmor\nvd^{Scer\UAS.T:Ivir\HA1}, Scer\GAL4^2-286 (Yoshiyama-Yanagawa et al., 2011, Yoshiyama-Yanagawa et al., 2011) Scer\GAL4^2-286, nvd^{dsRNA.IR-1.Scer\UAS} has lethal \| first instar larval stage phenotype, suppressible by Cele\daf-36^{Scer\UAS.T:Ivir\HA1}, Scer\GAL4^2-286 (Yoshiyama-Yanagawa et al., 2011, Yoshiyama-Yanagawa et al., 2011) Scer\GAL4^2-286, nvd^{dsRNA.IR-1.Scer\UAS} has lethal \| first instar larval stage phenotype, suppressible by Drer\nvd^{Scer\UAS.T:Ivir\HA1}, Scer\GAL4^2-286 (Yoshiyama-Yanagawa et al., 2011, Yoshiyama-Yanagawa et al., 2011) Scer\GAL4^2-286, nvd^{dsRNA.IR-1.Scer\UAS} has lethal \| first instar larval stage phenotype, suppressible by Scer\GAL4^2-286/Xlae\nvd^{Scer\UAS.T:Ivir\HA1} (Yoshiyama-Yanagawa et al., 2011, Yoshiyama-Yanagawa et al., 2011)
NOT suppressed by
Statement Reference Scer\GAL4^2-286, nvd^{dsRNA.IR-1.Scer\UAS} has auxotroph phenotype, non-suppressible by Dpac\nvd^Scer\UAS.cLa, Scer\GAL4^2-286 (Lang et al., 2012, Lang et al., 2012) Scer\GAL4^2-286, nvd^{dsRNA.IR-1.Scer\UAS} has lethal \| first instar larval stage phenotype, non-suppressible by Bmor\nvd^{H190A.Scer\UAS.T:Ivir\HA1}, Scer\GAL4^2-286 (Yoshiyama-Yanagawa et al., 2011, Yoshiyama-Yanagawa et al., 2011) Scer\GAL4^2-286, nvd^{dsRNA.IR-1.Scer\UAS} has lethal \| first instar larval stage phenotype, non-suppressible by Bmor\nvd^{H282A.Scer\UAS.T:Ivir\HA1}, Scer\GAL4^2-286 (Yoshiyama-Yanagawa et al., 2011, Yoshiyama-Yanagawa et al., 2011)
Suppressor of
Statement Reference Bmor\nm-g^Scer\UAS.cNa/Scer\GAL4^2-286 is a suppressor of lethal \| recessive phenotype of sro¹ (Niwa et al., 2010) Bmor\nvd^{Scer\UAS.T:Ivir\HA1}, Scer\GAL4^2-286 is a suppressor of lethal \| first instar larval stage phenotype of Scer\GAL4^2-286, nvd^{dsRNA.IR-1.Scer\UAS} (Yoshiyama-Yanagawa et al., 2011, Yoshiyama-Yanagawa et al., 2011) Cele\daf-36^{Scer\UAS.T:Ivir\HA1}, Scer\GAL4^2-286 is a suppressor of lethal \| first instar larval stage phenotype of Scer\GAL4^2-286, nvd^{dsRNA.IR-1.Scer\UAS} (Yoshiyama-Yanagawa et al., 2011, Yoshiyama-Yanagawa et al., 2011) Dpac\nvd^{4mut.Scer\UAS}, Scer\GAL4^2-286 is a suppressor of auxotroph phenotype of Scer\GAL4^2-286, nvd^{dsRNA.IR-1.Scer\UAS} (Lang et al., 2012, Lang et al., 2012) Drer\nvd^{Scer\UAS.T:Ivir\HA1}, Scer\GAL4^2-286 is a suppressor of lethal \| first instar larval stage phenotype of Scer\GAL4^2-286, nvd^{dsRNA.IR-1.Scer\UAS} (Yoshiyama-Yanagawa et al., 2011, Yoshiyama-Yanagawa et al., 2011) Scer\GAL4^2-286/Xlae\nvd^{Scer\UAS.T:Ivir\HA1} is a suppressor of lethal \| first instar larval stage phenotype of Scer\GAL4^2-286, nvd^{dsRNA.IR-1.Scer\UAS} (Yoshiyama-Yanagawa et al., 2011, Yoshiyama-Yanagawa et al., 2011)
NOT Suppressor of
Statement Reference Bmor\nvd^{H190A.Scer\UAS.T:Ivir\HA1}, Scer\GAL4^2-286 is a non-suppressor of lethal \| first instar larval stage phenotype of Scer\GAL4^2-286, nvd^{dsRNA.IR-1.Scer\UAS} (Yoshiyama-Yanagawa et al., 2011, Yoshiyama-Yanagawa et al., 2011) Bmor\nvd^{H282A.Scer\UAS.T:Ivir\HA1}, Scer\GAL4^2-286 is a non-suppressor of lethal \| first instar larval stage phenotype of Scer\GAL4^2-286, nvd^{dsRNA.IR-1.Scer\UAS} (Yoshiyama-Yanagawa et al., 2011, Yoshiyama-Yanagawa et al., 2011) Dpac\nvd^Scer\UAS.cLa, Scer\GAL4^2-286 is a non-suppressor of auxotroph phenotype of Scer\GAL4^2-286, nvd^{dsRNA.IR-1.Scer\UAS} (Lang et al., 2012, Lang et al., 2012)
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement Reference
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Rescues	Scer\GAL4^2-286/dib^Scer\UAS.cOa rescues dib^P3/dib^F8 (Wang et al., 2011) Scer\GAL4^2-286/Npc1a^Scer\UAS.cFa rescues Npc1a^57A (Fluegel et al., 2006) Scer\GAL4^2-286/sro^Scer\UAS.cNa rescues sro¹ (Niwa et al., 2010)
Partially rescues	Scer\GAL4^2-286/Npc1a^{Scer\UAS.T:Avic\GFP-EYFP} partially rescues Npc1a¹ (Huang et al., 2005, Phillips et al., 2008) Scer\GAL4^2-286/Npc1a^{Scer\UAS.T:Avic\GFP-EYFP} partially rescues Npc1a² (Huang et al., 2005) Scer\GAL4^2-286/Npc1a^{Scer\UAS.T:Avic\GFP-EYFP} partially rescues Npc1a^unspecified (Wang et al., 2011)
Fails to rescue	Scer\GAL4^2-286/Ns3^{Scer\UAS.T:Avic\GFP-YFP.Venus} fails to rescue Ns3^G0431 (Kaplan et al., 2008)
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 2 )
Reported As
Symbol Synonym	GAL4^2-286 Scer\GAL4^2-286
Name Synonym
Secondary FlyBase IDs

References ( 12 )
Research paper	Lang et al., 2012, Science 337(6102): 1658--1661 Mutations in the neverland gene turned Drosophila pachea into an obligate specialist species. [FBrf0219507] Wang et al., 2011, Dev. Biol. 351(1): 146--155 The cholesterol trafficking protein NPC1 is required for Drosophila spermatogenesis. [FBrf0212972] Yoshiyama-Yanagawa et al., 2011, J. Biol. Chem. 286(29): 25756--25762 The Conserved Rieske Oxygenase DAF-36/Neverland Is a Novel Cholesterol-metabolizing Enzyme. [FBrf0214362] Niwa et al., 2010, Development 137(12): 1991--1999 Non-molting glossy/shroud encodes a short-chain dehydrogenase/reductase that functions in the 'Black Box' of the ecdysteroid biosynthesis pathway. [FBrf0210924] Kaplan et al., 2008, Genes Dev. 22(14): 1877--1893 A nucleostemin family GTPase, NS3, acts in serotonergic neurons to regulate insulin signaling and control body size. [FBrf0205884] Phillips et al., 2008, J. Neurosci. 28(26): 6569--6582 Neuronal loss of Drosophila NPC1a causes cholesterol aggregation and age-progressive neurodegeneration. [FBrf0205488] Huang et al., 2007, Development 134(20): 3733--3742 Drosophila Niemann-Pick Type C-2 genes control sterol homeostasis and steroid biosynthesis: a model of human neurodegenerative disease. [FBrf0201424] Fluegel et al., 2006, Genetics 172(1): 185--196 Mutations of a Drosophila NPC1 gene confer sterol and ecdysone metabolic defects. [FBrf0190720] Yoshiyama et al., 2006, Development 133(13): 2565--2574 Neverland is an evolutionally conserved Rieske-domain protein that is essential for ecdysone synthesis and insect growth. [FBrf0193132] Huang et al., 2005, Development 132(22): 5115--5124 A Drosophila model of the Niemann-Pick type C lysosome storage disease: dnpc1a is required for molting and sterol homeostasis. [FBrf0190297] Andrews et al., 2002, Traffic 3(12): 906--921 Drosophila Sec10 is required for hormone secretion but not general exocytosis or neurotransmission. [FBrf0156063] Timmons et al., 1997, Dev. Genet. 20(4): 338--347 Green fluorescent protein/-galactosidase double reporters for visualizing Drosophila gene expression patterns. [FBrf0096427]

Allele Scer\GAL42-286

Allele Scer\GAL4^2-286