FlyBase Allele Report: Dmel\Trf2[G0071]

General Information

Symbol

Dmel\Trf2^G0071

Species

D. melanogaster

Name

FlyBase ID

FBal0096834

Feature type

allele

Associated gene

Dmel\Trf2

Associated Insertion(s)

P{lacW}Trf2^G0071

Carried in Construct

Also Known As

l(1)G0071

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(Schäfer et al., 1999.3.29)

Progenitor genotype

Associated Insertion(s)

P{lacW}Trf2^G0071

Cytology

Description

Maps approximately halfway through the Trf2 locus.

(Bashirullah et al., 2007)

Allele components

Component

Use(s)

Inserted element

P{lacW}

enhancer trap

Encoded product / tool

lacZ

reporter enzyme

Tagged with

Tag:NLS(P)

nuclear protein localization tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

X:8,420,676..8,420,852 [+]

(Bashirullah et al., 2007, Peter et al., 2002, Schäfer et al., 1999.3.29, Schäfer et al., 1999.6.15)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

AJ426786

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal | adult stage

(Peter et al., 2002)

lethal | pharate adult stage

(Peter et al., 2002)

lethal | recessive

(Schäfer et al., 1999.3.29)

lethal - all die before end of first instar larval stage

(Bashirullah et al., 2007)

lethal - all die before end of P-stage

(Wang et al., 2008)

lethal - all die during P-stage

(Fan et al., 2017)

some die during first instar larval stage

(Bashirullah et al., 2007)

some die during P-stage

(Wang et al., 2008)

Phenotype Manifest In

Detailed Description

Statement

Reference

The size of large lipid droplets in the fat body of Trf2^G0071 homozygous mutant larvae (second, early third as well as wandering third instar larval stage) is significantly increased compared to controls.

(Fan et al., 2017)

Mutants show defects in destruction of the larval salivary glands, having persistant larval salivary glands at the pupal stage.

(Wang et al., 2008)

Homozygous Trf2^G0071 are lethal, with majority of lethality occurring during the first instar larval stage. Those surviving to early pupae show defects in head eversion. No embryonic lethality is observed.

(Bashirullah et al., 2007)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Suppressed by

Statement

Reference

Trf2^G0071 has embryonic/larval fat body | wandering third instar larval stage phenotype, suppressible by Scer\GAL4^ppl.PP/Nepl5^UAS.Tag:FLAG

(Fan et al., 2017)

Trf2^G0071 has lipid droplet | wandering third instar larval stage phenotype, suppressible by Scer\GAL4^ppl.PP/Nepl5^UAS.Tag:FLAG

(Fan et al., 2017)

Trf2^G0071 has embryonic/larval fat body | wandering third instar larval stage phenotype, suppressible by eIF2Bδ^UAS.Tag:FLAG/Scer\GAL4^ppl.PP

(Fan et al., 2017)

Trf2^G0071 has lipid droplet | wandering third instar larval stage phenotype, suppressible by eIF2Bδ^UAS.Tag:FLAG/Scer\GAL4^ppl.PP

(Fan et al., 2017)

Trf2^G0071 has embryonic/larval fat body | wandering third instar larval stage phenotype, suppressible by CG2617^UAS.Tag:FLAG/Scer\GAL4^ppl.PP

(Fan et al., 2017)

Trf2^G0071 has lipid droplet | wandering third instar larval stage phenotype, suppressible by CG2617^UAS.Tag:FLAG/Scer\GAL4^ppl.PP

(Fan et al., 2017)

Trf2^G0071 has embryonic/larval fat body | wandering third instar larval stage phenotype, suppressible by Scer\GAL4^ppl.PP/AANATL2^UAS.Tag:FLAG

(Fan et al., 2017)

Trf2^G0071 has lipid droplet | wandering third instar larval stage phenotype, suppressible by Scer\GAL4^ppl.PP/AANATL2^UAS.Tag:FLAG

(Fan et al., 2017)

NOT suppressed by

Statement

Reference

Trf2^G0071 has embryonic/larval fat body | wandering third instar larval stage phenotype, non-suppressible by Acox3^UAS.Tag:FLAG/Scer\GAL4^ppl.PP

(Fan et al., 2017)

Trf2^G0071 has lipid droplet | wandering third instar larval stage phenotype, non-suppressible by Acox3^UAS.Tag:FLAG/Scer\GAL4^ppl.PP

(Fan et al., 2017)

Trf2^G0071 has embryonic/larval fat body | wandering third instar larval stage phenotype, non-suppressible by CG4586^UAS.Tag:FLAG/Scer\GAL4^ppl.PP

(Fan et al., 2017)

Trf2^G0071 has lipid droplet | wandering third instar larval stage phenotype, non-suppressible by CG4586^UAS.Tag:FLAG/Scer\GAL4^ppl.PP

(Fan et al., 2017)

Trf2^G0071 has embryonic/larval fat body | wandering third instar larval stage phenotype, non-suppressible by Scer\GAL4^ppl.PP/Taf12L^UAS.Tag:FLAG

(Fan et al., 2017)

Trf2^G0071 has lipid droplet | wandering third instar larval stage phenotype, non-suppressible by Scer\GAL4^ppl.PP/Taf12L^UAS.Tag:FLAG

(Fan et al., 2017)

Additional Comments

Genetic Interactions

Statement

Reference

The increased size of fat body lipid droplets characteristic for Trf2^G0071 mutant wandering third instar larvae is not significantly altered by expression of CG9527^{Scer\UAS.T:Zzzz\FLAG} or CG4586^{Scer\UAS.T:Zzzz\FLAG} or Taf12L^{Scer\UAS.T:Zzzz\FLAG} but is significantly rescued by expression of any of the following: CG2617^{Scer\UAS.T:Zzzz\FLAG}, AANATL2^{Scer\UAS.T:Zzzz\FLAG}, CG9507^{Scer\UAS.T:Zzzz\FLAG} or eIF2Bδ^{Scer\UAS.T:Zzzz\FLAG} under the control of Scer\GAL4^ppl.PP.

(Fan et al., 2017)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Trf2^G0071 is rescued by Trf2^UAS.s/Scer\GAL4^ppl.PP

(Fan et al., 2017)

Trf2^G0071 is rescued by Scer\GAL4^ppl.PP/Trf2^UAS.l

(Fan et al., 2017)

Comments

The increased size of fat body lipid droplets characteristic for Trf2^G0071 mutant wandering third instar larvae can be rescued by expression of either Trf2^Scer\UAS.s or Trf2^Scer\UAS.l under the control of Scer\GAL4^ppl.PP in the mutant background (which on its own leads to reduced lipid droplet size).

(Fan et al., 2017)

Images (0)

Mutant

Wild-type

Stocks (1)

Kyoto

111780

y¹ w^* P{lacW}Trf2^G0071 P{neoFRT}19A/FM7c; P{ey-FLP.N}5

Notes on Origin

Discoverer

Comments

P{lacW} not verified as causing the lethality.

(Schäfer et al., 1999.3.29)

External Crossreferences and Linkouts ( 1 )

Crossreferences

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

AJ426786

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

Trf2^G0071

(Neves and Eisenman, 2019)

dTrf2^G0071

(Bashirullah et al., 2007)

l(1)G0071

(Cherezov et al., 2020, Wang et al., 2008, Peter et al., 2002)

l(1)G0071^G0071

(Popodi et al., 2010-)

trf2^G0071

(Fan et al., 2017)

Name Synonyms

Secondary FlyBase IDs

References (9)

Report Sections

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