FB2025_01 , released February 20, 2025
Allele: Dmel\ci5m.UAS.Tag:HA
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General Information
Symbol
Dmel\ci5m.UAS.Tag:HA
Species
D. melanogaster
Name
FlyBase ID
FBal0102789
Feature type
allele
Associated gene
Dmel\ci
Associated Insertion(s)
Carried in Construct
P{UAS-ci.5m.HA}
Key Links
Transgenic product class
missense_variant
(Price and Kalderon, 1999)
polypeptide_post_translational_processing_variant
(Price and Kalderon, 1999)
Mutagen
Nature of the Allele
Transgenic product class
missense_variant
(Price and Kalderon, 1999)
polypeptide_post_translational_processing_variant
(Price and Kalderon, 1999)
Mutagen
in vitro construct
(Price and Kalderon, 1999)
Progenitor genotype
Carried in construct
P{UAS-ci.5m.HA}
Cytology
Description

A mutated form of ci (with mutations in the potential PKA phosphorylation sites 1, 2, 3, A and B), tagged at the N-terminal end with Tag:HA is expressed under the control of UASt regulatory sequences. The amino acid changes are S838A, S855A, S856A, S891A, S892A, S962A and T1006A.

(Price and Kalderon, 1999)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
ci
Tagged with
Tag:HA
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression of ci5m.Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL471B results in expansion of the wing disc in the wing blade region, with the anterior and posterior compartments being equally affected. Percentage embryonic lethality in lines expressing ciScer\UAS.cPa under the control of various Scer\GAL4 driver lines: 44% (Scer\GAL4e22c driver), 88% (Scer\GAL4ptc-559.1 driver) and 65% (Scer\GAL4en-e16E driver). Percentage larval/pupal lethality in lines expressing ciScer\UAS.cPa under the control of Scer\GAL471B : 77%.

      (Price and Kalderon, 1999)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      NOT suppressed by
      Statement
      Reference

      Scer\GAL471B, ci5m.UAS.Tag:HA has wing disc phenotype, non-suppressible by Mmus\PrkacamC.UAS, Scer\GAL471B

      (Price and Kalderon, 1999)
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference

      The wing disc phenotype caused by expression of ci5m.Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL471B phenotype is still seen if the animals are also co-expressing Mmus\PkacamC.Scer\UAS.

      (Price and Kalderon, 1999)
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      ci5m.Scer\UAS.T:Ivir\HA1
      ci5m.UAS.Tag:HA
      Name Synonyms
      Secondary FlyBase IDs
        References (2)