FB2025_01 , released February 20, 2025
Allele: Dmel\Lkb1S535A.UASp.GFP
Open Close
General Information
Symbol
Dmel\Lkb1S535A.UASp.GFP
Species
D. melanogaster
Name
FlyBase ID
FBal0141048
Feature type
allele
Associated gene
Dmel\Lkb1
Associated Insertion(s)
Carried in Construct
P{UASp-GFP-Lkb1.S535A}
Key Links
Transgenic product class
missense_variant
(Martin and St. Johnston, 2003)
polypeptide_post_translational_processing_variant
(Martin and St. Johnston, 2003)
Mutagen
Nature of the Allele
Transgenic product class
missense_variant
(Martin and St. Johnston, 2003)
polypeptide_post_translational_processing_variant
(Martin and St. Johnston, 2003)
Mutagen
in vitro construct
(Martin and St. Johnston, 2003)
Progenitor genotype
Carried in construct
P{UASp-GFP-Lkb1.S535A}
Cytology
Description

UASp regulatory sequences control expression of a 3.6kb genomic fragment that contains the Lkb1 transcription unit, mutated to contain a modification (S535A) that prevents phosphorylation of the gene product by Pka-C1 when expressed in S2 cells. A GFP tag has been inserted at the N-terminal end of the Lkb1 open reading frame.

(Martin and St. Johnston, 2003)
Allele components
Component
Use(s)
Regulatory region(s)
UASp
Encoded product / tool
Lkb1
Tagged with
GFP
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Partially rescues

      Lkb1S535A.UASp.GFP/Scer\GAL4VP16.mat.αTub67C partially rescues Lkb14B1-11/Lkb14A4-2

      (Martin and St. Johnston, 2003)

      Scer\GAL4arm.PS/Lkb1S535A.UASp.GFP partially rescues Lkb14B1-11/Lkb14A4-2

      (Martin and St. Johnston, 2003)
      Comments

      Expression of lkb1S535A.Scer\UAS.P\T.T:Avic\GFP under the control of Scer\GAL4mat.αTub67C.T:Hsim\VP16 partially rescues the loss of polarity in lkb14A4-2 germ line clone oocytes. lkb1S535A.Scer\UAS.P\T.T:Avic\GFP; Scer\GAL4arm.PS rescues the lethality due to lkb14A4-2/lkb14B1-11. The resulting flies have ovaries with a disorganised follicular epithelium in which most cells appear unpolarized, and loss of anterior-posterior polarisation of oocytes.

      (Martin and St. Johnston, 2003)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (4)
      Reported As
      Symbol Synonym
      Lkb1S535A.Scer\UAS.P\T.T:Avic\GFP
      Lkb1S535A.UASp.GFP
      lkb1S535A.Scer\UAS.P\T.T:Avic\GFP
      lkb1S535A.Ser\UAS.P\T.T:Avic\GFP
      Name Synonyms
      Secondary FlyBase IDs
        References (1)