FlyBase Allele Report: Dmel\hang[AE10]

General Information

Symbol

Dmel\hang^AE10

Species

D. melanogaster

Name

FlyBase ID

FBal0155213

Feature type

allele

Associated gene

Dmel\hang

Associated Insertion(s)

P{PZ}hang^AE10

Carried in Construct

Key Links

Allele class

Mutagen

Nature of the Allele

Allele class

Mutagen

Progenitor genotype

Associated Insertion(s)

P{PZ}hang^AE10

Cytology

Description

P{PZ} insertion in the first exon of hang, in the coding region.

(Scholz et al., 2005)

Allele components

Component

Use(s)

Inserted element

P{PZ}

enhancer trap

Encoded product / tool

lacZ

reporter enzyme

Tagged with

Tag:NLS(P)

nuclear protein localization tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of alcohol use disorder

CEA

(Scholz et al., 2005)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

model of alcohol use disorder

is exacerbated by Tbh^nM18

(Scholz et al., 2005)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal behavior | adult stage

(Velo Escarcena et al., 2021)

chemical sensitive

(Scholz et al., 2005, Schwenkert et al., 2003)

chemical sensitive | adult stage

(Ruppert et al., 2017)

short lived

(Scholz et al., 2005)

viable

(Scholz et al., 2005)

Phenotype Manifest In

Detailed Description

Statement

Reference

hang^AE10 adult mutants exhibit reduced development of tolerance to ethanol exposure (increase in resistance upon re-exposition) as well as decreased heat-induced ethanol resistance normally observed in wild-type flies.

(Ruppert et al., 2017)

hang^AE10 mutant flies show a normal initial mean elution time (MET) in an inebriometer, but have a reduced ability to develop tolerance to ethanol vapour compared to controls (14 +/- 3% increase in MET after pre-exposure to ethanol compared to an increase of 35 +/- 2% for control flies). The mutant flies also show defective ethanol tolerance in a chronic tolerance protocol in which flies are exposed to ethanol in the inebriometer four times at 2 hour intervals. Control flies which are preexposed to a heat shock show a 46 +/- 4% increase in mean elution time (MET) when exposed to ethanol in an inebriometer 4 hours later. This heat-ethanol cross tolerance is substantially reduced in hang^AE10 flies (only a 26 +/- 4% increase in MET is seen). Mutant flies show a decreased lifespan under control conditions and show increased sensitivity to paraquat compared to controls. The overall brain structure of mutant adults appears to be normal, even in old flies.

(Scholz et al., 2005)

Molecular genetic and phenotypic characterizations suggest that hang^AE10 mutants are impaired in specific cellular stress responses.

(Schwenkert et al., 2003)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhanced by

Statement

Reference

hang^AE10 has chemical sensitive | adult stage phenotype, non-enhanceable by Pde4^Δ143

(Ruppert et al., 2017)

Suppressed by

Statement

Reference

hang^AE10 has chemical sensitive | adult stage phenotype, suppressible by Pde4^Δ143

(Ruppert et al., 2017)

Suppressor of

Statement

Reference

hang^AE10 is a suppressor of chemical sensitive | adult stage phenotype of Pde4^Δ143

(Ruppert et al., 2017)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

The reduced heat-induced ethanol (EtOH) resistance characteristic for hang^AE10 mutant adults is not exacerbated further by combination with dnc^Δ143 which on its own also lead to a decrease in the heat > ethanol cross-tolerance.

hang^AE10;dnc^Δ143 double mutant adult flies display normal ethanol tolerance development upon re-exposition to EtOH, unlike each of the single mutants that show significantly reduced tolerance buildout.

(Ruppert et al., 2017)

Ethanol tolerance is almost completely abolished in hang^AE10 Tbh^nM18 double mutant flies.

(Scholz et al., 2005)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

hang^AE10 is rescued by Scer\GAL4^Pde4.RA/hang^UAS.cSa

(Ruppert et al., 2017)

hang^AE10 is rescued by Scer\GAL4^NP6510/hang^UAS.cSa

(Ruppert et al., 2017)

hang^AE10 is rescued by Scer\GAL4^Appl.G1a/hang^UAS.cSa

(Scholz et al., 2005)

Comments

The reduced ability of hang^AE10 mutant adults to develop tolerance to ethanol upon re-exposition is rescued by expression of hang^Scer\UAS.cSa under the control of either Scer\GAL4^dnc.RA or Scer\GAL4^NP6510 together with tub-Gal80[ts] to limit the expression to a short period before the inebriation test (temporally unrestricted expression of hang^Scer\UAS.cSa in the mutant background using either of the two drivers is lethal).

(Ruppert et al., 2017)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Comments

Precise excision of the P{PZ} element reverts the mutant phenotype.

(Scholz et al., 2005)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

hang^AE10

(Velo Escarcena et al., 2021, Ruppert et al., 2017)

hang^AE10NT

Name Synonyms

hangoverAE10NT

(Lindsay et al., 2008, Scholz and Heberlein, 2004)

Secondary FlyBase IDs

References (6)

Report Sections

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