FB2025_01 , released February 20, 2025
Allele: Dmel\AkhUAS.cLa
Open Close
General Information
Symbol
Dmel\AkhUAS.cLa
Species
D. melanogaster
Name
Saccharomyces cerevisiae UAS construct a of Lee
FlyBase ID
FBal0157367
Feature type
allele
Associated gene
Dmel\Akh
Associated Insertion(s)
Carried in Construct
P{UAS-Akh.L}
Also Known As
UAS-Akh, UAS-dAkh
Key Links
Transgenic product class
cDNA
(Lee and Park, 2004)
wild_type
(Lee and Park, 2004)
Mutagen
Nature of the Allele
Transgenic product class
cDNA
(Lee and Park, 2004)
wild_type
(Lee and Park, 2004)
Mutagen
in vitro construct
(Lee and Park, 2004)
Progenitor genotype
Carried in construct
P{UAS-Akh.L}
Cytology
Description

UASt regulatory sequences drive expression of an Akh cDNA.

(Lee and Park, 2004)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
Akh
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Overexpression of AkhScer\UAS.cLa driven by Scer\GAL4Akh.PL significantly decreases survival under starvation conditions, compared to controls.

      (Hentze et al., 2015)

      Mid-third instar larvae expressing AkhScer\UAS.cLa under the control of Scer\GAL4Act.PU exhibit moderately increased glucose levels.

      (Ugrankar et al., 2015)

      Expression of AkhScer\UAS.cLa under the control of Scer\GAL4fat largely depletes lipid storage droplets and organismal fat stores.

      (Grönke et al., 2007)

      Expression of AkhScer\UAS.cLa under the control of Scer\GAL4Akh.PL does not alter larval hemolymph trehalose levels. Expression of AkhScer\UAS.cLa under the control of Scer\GAL4r4 results in larval hemolymph trehalose levels that are about 34% above normal. In addition, hemolymph glycerol concentrations are significantly higher than normal, while the triglyceride content of the fat body is reduced.

      (Lee and Park, 2004)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      NOT Suppressor of
      Statement
      Reference

      AkhUAS.cLa, Scer\GAL4Akh.PL is a non-suppressor of long lived | nutrition conditional phenotype of AstA-R2GD339, Scer\GAL4Akh.PL

      (Hentze et al., 2015)
      Phenotype Manifest In
      NOT Suppressor of
      Statement
      Reference

      Scer\GAL4fat/AkhUAS.cLa is a non-suppressor of lipid droplet phenotype of AkhR1

      (Grönke et al., 2007)
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Expression of AkhScer\UAS.cLa under the control of Scer\GAL4fat has no effect on the increased organismal fat content and accumulation of lipid storage droplets seen in GRHR1 mutant flies.

      Expression of AkhScer\UAS.cLa under the control of Scer\GAL4fat reduces the increased organismal fat content seen in bmm1 mutant flies.

      (Grönke et al., 2007)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Partially rescues

      Scer\GAL4Act.PU/AkhUAS.cLa partially rescues Akh1

      (Sajwan et al., 2015)
      Comments

      Ectopic expression of AkhScer\UAS.cLa under the control of Scer\GAL4Act.PU in the Akh1 mutant background suppresses the increased survival rate of Akh1 adult flies under starvation: the survival rate of AkhScer\UAS.cLa expressing flies is significantly lower than in both Akh1 mutants and wild-type controls.

      (Sajwan et al., 2015)
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      AkhScer\UAS.cLa
      AkhUAS.cLa
      Name Synonyms
      Saccharomyces cerevisiae UAS construct a of Lee
      (Lee and Park, 2004)
      Secondary FlyBase IDs
        References (15)