FB2025_01 , released February 20, 2025
Allele: Dmel\Adar3.4.G.UAS
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General Information
Symbol
Dmel\Adar3.4.G.UAS
Species
D. melanogaster
Name
FlyBase ID
FBal0190679
Feature type
allele
Associated gene
Dmel\Adar
Associated Insertion(s)
Carried in Construct
P{UAS-Adar.3.4.G}
Key Links
Transgenic product class
missense_variant
(Keegan et al., 2005)
Mutagen
Nature of the Allele
Transgenic product class
missense_variant
(Keegan et al., 2005)
Mutagen
in vitro construct
(Keegan et al., 2005)
Progenitor genotype
Adar3.4.UAS
(Keegan et al., 2005)
Carried in construct
P{UAS-Adar.3.4.G}
Cytology
Description

UASt regulatory sequences drive expression of cDNA encoding the Adar 3/4 isoform with a Ser to Gly mutation in exon 7, close to the active site (codon is changed from AGT to GGT). This mutation is in a codon that can normally undergo RNA editing (carried out by the Adar protein), and the protein produced corresponds to the form produced after RNA editing has occurred. The coding region is tagged with both Tag:FLAG and Tag:polyHis.

(Keegan et al., 2005)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
Adar
Tagged with
Tag:FLAG
Tag:polyHis
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Among male progeny of rescue crosses that carry both the Adar1F4 mutation and the Adar3.4.G.Scer\UAS construct, the number of Scer\GAL4Act5C.PI driver flies obtained, expressed as a percentage of the number of control flies lacking a GAL4 driver, is 69%.

      (Keegan et al., 2005)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Partially rescues

      Scer\GAL4Act5C.PI/Adar3.4.G.UAS partially rescues Adar1F4

      (Keegan et al., 2005)
      Comments

      Rescue of the Adar1F4 locomotion defect with the Adar3.4.G.Scer\UAS construct is partial.

      (Keegan et al., 2005)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      Adar3.4.G.Scer\UAS
      Adar3.4.G.UAS
      Name Synonyms
      Secondary FlyBase IDs
        References (1)